| Metallothioneins Protect Cytosolic Creatine kinases Against Stress Induced by Nitrogen-Based Oxidants. | |
| | |
MedLine Citation:
|
PMID: 21967612 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The formation of intracellular nitrogen-based oxidants has important physiological and pathological consequences. Creatine kinase (CK), which plays a key role in intracellular energy metabolism, is a main target of low concentrations of oxidative and nitrative stresses. In this research, the interaction between cytosolic CKs (MM-CK and BB-CK) and metallothioneins (hMT2A and hMT3) were characterized by both in vitro and in intact cell assays. MTs could successfully protect the cytosolic CKs against inactivation induced by low concentrations of peroxynitrite (PN) and nitric oxide both in vitro and in hMT2A over-expressing H9c2 cells and hMT3 knock-down U-87 MG cells. Under high PN concentrations, CK formed granule-like structures, and MTs were well co-localized in these aggregated granules. Further analysis indicated that the number of the cells containing the CK aggregates negatively correlated to the expression levels of MTs. In vitro experiments indicated that MTs could effectively protect CKs against aggregation during refolding, suggesting that MT might function as a chaperone to assist CK reactivation. The findings herein provide direct evidence of the connection between the two well characterized intracellular systems: the precisely balanced energy homeostasis by CKs and the oxidative-stress response system using MTs. |
| | |
Authors:
|
Zhe Chen; Jie Li; Tong-Jin Zhao; Xu-Hui Li; Fan-Guo Meng; Hang Mu; Yong-Bin Yan; Hai-Meng Zhou |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-10-3 |
Journal Detail:
|
Title: The Biochemical journal Volume: - ISSN: 1470-8728 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-10-4 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2984726R Medline TA: Biochem J Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Genetic Modifiers of Sickle Cell Disease.
Next Document: Congenitally Corrected Transposition of the Great Arteries with Severely Stenotic Bicuspid Aortic Va...