Document Detail

Metallothionein-3 is expressed in the brain and various peripheral organs of the rat.
MedLine Citation:
PMID:  18479819     Owner:  NLM     Status:  MEDLINE    
Metallothionein-3 (MT-3), also known as growth inhibitory factor (GIF), was originally identified in the brain. An essential step in elucidating the potential roles of MT-3 is to evaluate its expression levels in organs other than the brain. In this present study, we carried out RT-PCR, Western blot and immunohistochemical analyses to quantify MT-3 mRNA and its protein in the cerebrum, eye, heart, kidney, liver, prostate, testis, tongue, and muscle in male Wistar rats. MT-3 mRNA was detected in the cerebrum, the dorsolateral lobe of the prostate, testis, and tongue. Using a monoclonal anti-MT-3 antibody, we detected MT-3 in the cerebrum, the dorsolateral lobe of the prostate, testis, and tongue as a single band on an immunoblot. Immunohistochemical staining showed MT-3 in some astrocytes in the deep cortex, ependymal cells, and choroidal cells in the cerebrum. MT-3 was also detected in some cells of the glomerulus and the collective tubules in the kidney, some cells in the glandular epithelium of the dorsolateral lobe of the prostate, some Sertoli cells and Lydig cells in the testis, and taste bud cells in the tongue. Although MT-3 immunopositivity was obviously demonstrated in the kidney by the immnunohistochemical method, the expression of MT-3 was not fully detectable by RT-PCR and Western blot analyses. Interestingly, only a subset of cells showed positivity for MT-3, not all cells in all tissues. The localization of MT-3 in peripheral organs outside the brain suggests that MT-3 has roles in these tissues besides its role in growth inhibition of neurites.
Isao Hozumi; Junko S Suzuki; Hiroaki Kanazawa; Akira Hara; Masanao Saio; Takashi Inuzuka; Shinichi Miyairi; Akira Naganuma; Chiharu Tohyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-18
Journal Detail:
Title:  Neuroscience letters     Volume:  438     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-23     Completed Date:  2008-09-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  54-8     Citation Subset:  IM    
Department of Neurology and Geriatrics, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
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MeSH Terms
Astrocytes / cytology,  metabolism
Blotting, Western
Brain / anatomy & histology,  enzymology*
Choroid Plexus / cytology,  metabolism
Ependyma / cytology,  metabolism
Epithelial Cells / cytology,  metabolism*
Growth Inhibitors / metabolism
Kidney / cytology,  metabolism
Nerve Tissue Proteins / analysis,  genetics,  metabolism*
Organ Specificity
Prostate / cytology,  metabolism
RNA, Messenger / analysis,  metabolism
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Taste Buds / cytology,  metabolism
Testis / cytology,  metabolism
Tongue / cytology,  metabolism
Viscera / cytology,  enzymology*
Reg. No./Substance:
0/Growth Inhibitors; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/growth inhibitory factor

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