| Metallothionein-3 is expressed in the brain and various peripheral organs of the rat. | |
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MedLine Citation:
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PMID: 18479819 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Metallothionein-3 (MT-3), also known as growth inhibitory factor (GIF), was originally identified in the brain. An essential step in elucidating the potential roles of MT-3 is to evaluate its expression levels in organs other than the brain. In this present study, we carried out RT-PCR, Western blot and immunohistochemical analyses to quantify MT-3 mRNA and its protein in the cerebrum, eye, heart, kidney, liver, prostate, testis, tongue, and muscle in male Wistar rats. MT-3 mRNA was detected in the cerebrum, the dorsolateral lobe of the prostate, testis, and tongue. Using a monoclonal anti-MT-3 antibody, we detected MT-3 in the cerebrum, the dorsolateral lobe of the prostate, testis, and tongue as a single band on an immunoblot. Immunohistochemical staining showed MT-3 in some astrocytes in the deep cortex, ependymal cells, and choroidal cells in the cerebrum. MT-3 was also detected in some cells of the glomerulus and the collective tubules in the kidney, some cells in the glandular epithelium of the dorsolateral lobe of the prostate, some Sertoli cells and Lydig cells in the testis, and taste bud cells in the tongue. Although MT-3 immunopositivity was obviously demonstrated in the kidney by the immnunohistochemical method, the expression of MT-3 was not fully detectable by RT-PCR and Western blot analyses. Interestingly, only a subset of cells showed positivity for MT-3, not all cells in all tissues. The localization of MT-3 in peripheral organs outside the brain suggests that MT-3 has roles in these tissues besides its role in growth inhibition of neurites. |
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Authors:
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Isao Hozumi; Junko S Suzuki; Hiroaki Kanazawa; Akira Hara; Masanao Saio; Takashi Inuzuka; Shinichi Miyairi; Akira Naganuma; Chiharu Tohyama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-04-18 |
Journal Detail:
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Title: Neuroscience letters Volume: 438 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-23 Completed Date: 2008-09-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 54-8 Citation Subset: IM |
Affiliation:
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Department of Neurology and Geriatrics, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan. ihozumi@gifu-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Astrocytes / cytology, metabolism Blotting, Western Brain / anatomy & histology, enzymology* Choroid Plexus / cytology, metabolism Ependyma / cytology, metabolism Epithelial Cells / cytology, metabolism* Growth Inhibitors / metabolism Immunohistochemistry Kidney / cytology, metabolism Male Nerve Tissue Proteins / analysis, genetics, metabolism* Organ Specificity Prostate / cytology, metabolism RNA, Messenger / analysis, metabolism Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Taste Buds / cytology, metabolism Testis / cytology, metabolism Tongue / cytology, metabolism Viscera / cytology, enzymology* |
| Chemical | |
Reg. No./Substance:
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0/Growth Inhibitors; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/growth inhibitory factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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