| Metalloproteinases 2 and 9 activity during promotion and progression stages of rat liver carcinogenesis. | |
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MedLine Citation:
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PMID: 19052882 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Activity of metalloproteinases 2 and 9 (MMP-2 and 9) during promotion and progression of rat liver carcinogenesis was investigated in a modified resistant hepatocyte model. Development of preneoplastic liver lesions positive for glutathione S-transferase 7-7-(GST-P 7-7-positive PNL) and tumors besides hepatocytes positive for proliferating cell nuclear antigen (PCNA) were quantified and compared to MMP-2 and-9 activity using gelatin zymography. Marked increases in GST-P 7-7-positive PNL development, PCNA labeling indices, MMP-2 (pro, intermediate and active forms) and pro-MMP-9 activity were observed after proliferative stimulus induced by 2-acetylaminofluorene (2-AAF) exposure cycles. After 2-AAF withdrawal, increase in MMP-2 activity was detected only in neoplastic mixed lesions, whereas active MMP-9 was increased in both PLN and neoplastic tissues. Our findings suggest that MMP-2 may be associated with proliferative events induced by 2-AAF rather than with selective growth of PNL and that MMP-9 could be associated with progression of PNL and neoplastic mixed lesions. |
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Authors:
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Kelly Silva Furtado; Paulo Wagner Pires; Luis Antonio Justulin; Maria Aparecida Marchesan Rodrigues; Sergio Luis Felisbino; Luis Fernando Barbisan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-04 |
Journal Detail:
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Title: Journal of molecular histology Volume: 40 ISSN: 1567-2387 ISO Abbreviation: J. Mol. Histol. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-03-19 Completed Date: 2009-10-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101193653 Medline TA: J Mol Histol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-11 Citation Subset: IM |
Affiliation:
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School of Medicine, Department of Pathology, UNESP São Paulo State University, Botucatu, SP 18618-000, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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2-Acetylaminofluorene
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toxicity Animals Body Weight / drug effects Diethylnitrosamine / toxicity Disease Progression Glutathione S-Transferase pi / metabolism Immunohistochemistry Liver / drug effects, metabolism, pathology Liver Neoplasms, Experimental / chemically induced, metabolism*, pathology Male Matrix Metalloproteinase 2 / metabolism* Matrix Metalloproteinase 9 / metabolism* Organ Size / drug effects Precancerous Conditions / chemically induced, metabolism*, pathology Proliferating Cell Nuclear Antigen / metabolism Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Proliferating Cell Nuclear Antigen; 53-96-3/2-Acetylaminofluorene; 55-18-5/Diethylnitrosamine; EC 2.5.1.18/Glutathione S-Transferase pi; EC 2.5.1.18/Gstp1 protein, rat; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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