Document Detail

Metalloproteinase expression in normal and malignant oral keratinocytes: stimulation of MMP-2 and -9 by scatter factor.
MedLine Citation:
PMID:  10946762     Owner:  NLM     Status:  MEDLINE    
Matrix metalloproteinases (MMPs) are Zn2+ dependent proteases produced by a variety of cell types. They have a fundamental role in tissue remodelling, tumour invasion and metastasis. Scatter factor (SF), secreted by fibroblasts, has a paracrine action on epithelial cells and binds the trans-membrane c-met receptor inducing loss of adhesion, cell motility and invasiveness in vitro. The purpose of this study was to test if SF can regulate the production of MMPs by epithelial cells. Supernatants from oral squamous cell carcinoma-derived cells (H375 and H376), a human keratinocyte line (UP), and primary cultures of oral mucosal keratinocytes, grown in the presence or absence of SF, were analysed using 0.1% gelatin zymography. MMPs were characterised by comparison with human recombinant enzymes and by the use of specific inhibitors. Oral mucosal keratinocytes, UP, and H357 cells expressed MMP-2 and MMP-9, whilst H376 cells only expressed MMP-2. SF increased the expression of MMP-9 in UP and MMP-2 in H376 supernatants. Both MMP-2 and MMP-9 activity were increased in H357 and normal keratinocyte supernatants. This could be blocked using a human recombinant anti-SF antibody. In all epithelial lines tested, c-Met, the cell surface receptor for SF, could be detected. The results indicate that SF stimulates MMP expression in UP, H376, H357, and normal oral mucosal cells and points to a role for SF in the regulation of oral keratinocyte behaviour in wound healing and neoplasia.
J H Bennett; M J Morgan; S A Whawell; P Atkin; P Roblin; J Furness; P M Speight
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of oral sciences     Volume:  108     ISSN:  0909-8836     ISO Abbreviation:  Eur. J. Oral Sci.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-12-06     Completed Date:  2000-12-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9504563     Medline TA:  Eur J Oral Sci     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  281-91     Citation Subset:  D; IM    
Department of Oral Pathology, Eastman Dental Institute for Oral Health Care Sciences, University College London, UK.
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MeSH Terms
Analysis of Variance
Blotting, Western
Carcinoma, Squamous Cell / enzymology*
Cell Line, Transformed
Culture Media, Conditioned / pharmacology
Electrophoresis, Polyacrylamide Gel / methods
Enzyme Induction / drug effects
Gene Expression
Hepatocyte Growth Factor / pharmacology,  physiology*
Keratinocytes / enzymology*
Matrix Metalloproteinase 2 / biosynthesis
Matrix Metalloproteinase 9 / biosynthesis
Matrix Metalloproteinases / biosynthesis*
Mouth Mucosa / cytology,  enzymology
Mouth Neoplasms / enzymology*
Proto-Oncogene Proteins c-met / biosynthesis*
RNA, Messenger / analysis
Tumor Cells, Cultured / enzymology
Reg. No./Substance:
0/Culture Media, Conditioned; 0/RNA, Messenger; 67256-21-7/Hepatocyte Growth Factor; EC Proteins c-met; EC 3.4.24.-/Matrix Metalloproteinases; EC Metalloproteinase 2; EC Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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