Document Detail

Metalloproteinase 9 is the outer executioner of desmoglein 3 in apoptotic keratinocytes.
MedLine Citation:
PMID:  17448220     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate the specific matrix metalloproteinases (MMPs) targeting desmoglein 3 (Dsg3) in apoptotic keratinocytes. METHOD: Inhibitor studies on cultured keratinocytes and Western blot analysis. RESULTS: Blocking of MMP-9 activity strongly reduces shedding of Dsg3 from cell surface. MMP-2 has a less relevant role in the cleavage of Dsg3 while other MMPs, such as MMP-1, -3, and -8, do not target Dsg3. CONCLUSION: Apoptic keratinocytes impair the extracellular domain of cell surface Dsg3 by MMP-9 activity. The discovery of a specific targeting of Dsg3 could be useful to understand the pathophysiology of diseases in which Dsg3 is affected.
N Cirillo; F Femiano; F Gombos; A Lanza
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral diseases     Volume:  13     ISSN:  1354-523X     ISO Abbreviation:  Oral Dis     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-23     Completed Date:  2007-06-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508565     Medline TA:  Oral Dis     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  341-5     Citation Subset:  D    
Regional Center on Craniofacial Malformations-MRI, Department of Odontostomatology, 1st School of Medicine and Surgery, II University of Naples, Naples, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blotting, Western
Cell Line, Transformed
Desmoglein 3 / metabolism*
Enzyme Inhibitors / pharmacology
Keratinocytes / cytology,  drug effects,  enzymology*
Matrix Metalloproteinase 9 / metabolism*
Microscopy, Fluorescence
Reverse Transcriptase Polymerase Chain Reaction
Staurosporine / pharmacology
Reg. No./Substance:
0/Desmoglein 3; 0/Enzyme Inhibitors; 62996-74-1/Staurosporine; EC Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Oral leishmaniasis: a clinicopathological study of 11 cases.
Next Document:  A verification protocol for the probe sequences of Affymetrix genome arrays reveals high probe accur...