Document Detail

Metalloproteinase-9 in induced sputum correlates with the severity of the late allergen-induced asthmatic response.
MedLine Citation:
PMID:  15133340     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Asthma is characterized by airway inflammation and remodeling in which matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMP) play an important role. Allergen exposure activates the inflammatory/repair process in sensitized subjects. Induced-sputum analysis is a non-invasive method that allows the assessment of changes in inflammatory and remodeling mediators implicated in asthma. OBJECTIVES: To evaluate the changes in MMP-9 and its principal inhibitor (TIMP-1) in sputum and plasma of mild allergic asthmatic subjects after whole-lung allergen challenge. METHODS: Induced sputum and blood samples were obtained at baseline, and 6 and 24 h after challenge. MMP-9 and TIMP-1 levels in sputum and plasma were measured by ELISA. RESULTS: Allergen challenge increased the percentage of sputum eosinophils and MMP-9 levels 6 and 24 h after the challenge compared to baseline levels, but TIMP-1 levels did not vary significantly. A significant correlation was observed between MMP-9 levels at 6 h and the maximum percent fall in FEV(1) during the late response. Throughout the study, MMP-9 levels correlated significantly with the number of neutrophils in sputum. CONCLUSIONS: This study shows that analysis of induced sputum is a useful tool to study the variations in MMP-9 and TIMP-1 levels following allergen challenge, therefore allowing to evaluate their role in allergen-induced airway damage and repair.
Marie-Eve Boulay; Philippe Prince; Francine Deschesnes; Jamila Chakir; Louis-Philippe Boulet
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Respiration; international review of thoracic diseases     Volume:  71     ISSN:  0025-7931     ISO Abbreviation:  Respiration     Publication Date:    2004 May-Jun
Date Detail:
Created Date:  2004-05-10     Completed Date:  2004-10-12     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0137356     Medline TA:  Respiration     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  216-24     Citation Subset:  IM    
Copyright Information:
Copyright 2004 S. Karger AG, Basel
Centre de recherche de l'Hôpital Laval, Institut universitaire de cardiologie et de pneumologie, Université Laval, Sainte-Foy, Que., Canada.
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MeSH Terms
Allergens / adverse effects*
Asthma / immunology*
Matrix Metalloproteinase 9 / analysis*
Severity of Illness Index
Sputum / chemistry
Tissue Inhibitor of Metalloproteinase-1 / analysis
Reg. No./Substance:
0/Allergens; 0/Tissue Inhibitor of Metalloproteinase-1; EC Metalloproteinase 9

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