| Metalloprotease-dependent onset of blood circulation in zebrafish. | |
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MedLine Citation:
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PMID: 20605457 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The primitive blood circulation requires intravascular plasma flow. However, it remains unclear whether the onset of earliest blood circulation is dependent solely on establishment of a functional circulatory organ or whether it also requires active processes inherent in blood cells. In this study, we present novel mechanisms for the onset of blood circulation by monitoring fluorescently labeled blood precursors and blood vessels in zebrafish. The earliest blood circulation occurs synchronously. This synchrony is achieved by the retention of erythroid precursors on the lumen of the vasculature after their invasion from the subaortic region, and then by simultaneous release of these precursors into the flow. Morphological and biochemical analyses suggest that the onset of blood circulation accompanies disruption of blood cell-to-vessel adhesion and requires metalloprotease-dependent processes. ADAM8, a member of the a disintegrin and metalloprotease (ADAM) family, mediates the onset of blood circulation. In ADAM8-depleted embryos, erythroid cells fail to detach from the vascular lumen and stagnate. Expression of a protease-defective ADAM8 in erythroid cells causes dominant-negative effects on blood circulation, suggesting cell-autonomous roles of ADAM8. Based on these findings, we propose that the first erythroid cells require both flow-dependent passive and proteolysis-dependent active processes to enter the circulation. |
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Authors:
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Atsuo Iida; Kazuya Sakaguchi; Kiyoaki Sato; Hidetoshi Sakurai; Daigo Nishimura; Aya Iwaki; Miki Takeuchi; Makoto Kobayashi; Kazuyo Misaki; Shigenobu Yonemura; Atsuo Kawahara; Atsuko Sehara-Fujisawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-03 |
Journal Detail:
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Title: Current biology : CB Volume: 20 ISSN: 1879-0445 ISO Abbreviation: Curr. Biol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-11-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9107782 Medline TA: Curr Biol Country: England |
Other Details:
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Languages: eng Pagination: 1110-6 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Growth Regulation, Institute for Frontier Medical Sciences, Kyoto University, Kawahara-cho 53, Shogo-in, Kyoto 606-8507, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Circulation* Metalloproteases / metabolism* Zebrafish |
| Chemical | |
Reg. No./Substance:
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EC 3.4.-/Metalloproteases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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