Document Detail


Metal plasmon-coupled fluorescence imaging and label free coenzyme detection in cells.
MedLine Citation:
PMID:  22713456     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Flavin adenine dinucleotide (FAD) is a key metabolite in cellular energy conversion. Flavin can also bind with some enzymes in the metabolic pathway and the binding sites may be changed due to the disease progression. Thus, there is interest on studying its expression level, distribution, and redox state within the cells. FAD is naturally fluorescent, but it has a modest extinction coefficient and quantum yield. Hence the intrinsic emission from FAD is generally too weak to be isolated distinctly from the cellular backgrounds in fluorescence cell imaging. In this article, the metal nanostructures on the glass coverslips were used as substrates to measure FAD in cells. Particulate silver films were fabricated with an optical resonance near the absorption and the emission wavelengths of FAD which can lead to efficient coupling interactions. As a result, the emission intensity and quantum yield by FAD were greatly increased and the lifetime was dramatically shortened resulting in less interference from the longer lived cellular background. This feature may overcome the technical limits that hinder the direct observation of intrinsically fluorescent coenzymes in the cells by fluorescence microscopy. Fluorescence cell imaging on the metallic particle substrates may provide a non-invasive strategy for collecting the information of coenzymes in cells.
Authors:
Jian Zhang; Yi Fu; Ge Li; Richard Y Zhao
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-06-17
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  425     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-28     Completed Date:  2012-12-28     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  696-700     Citation Subset:  IM    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
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MeSH Terms
Descriptor/Qualifier:
Coenzymes / analysis
Flavin-Adenine Dinucleotide / analysis*
Fluorescence
HeLa Cells
Humans
Metal Nanoparticles*
Microscopy, Fluorescence
Molecular Imaging / methods*
Surface Plasmon Resonance / methods*
Grant Support
ID/Acronym/Agency:
CA134386/CA/NCI NIH HHS; EB006521/EB/NIBIB NIH HHS; EB009509/EB/NIBIB NIH HHS; HG-002655/HG/NHGRI NIH HHS; HG005090/HG/NHGRI NIH HHS; R01 EB006521/EB/NIBIB NIH HHS; R01 HG002655/HG/NHGRI NIH HHS; R21 CA134386/CA/NCI NIH HHS; R21 CA147975/CA/NCI NIH HHS; R21 EB009509/EB/NIBIB NIH HHS; R21 HG005090/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/Coenzymes; 146-14-5/Flavin-Adenine Dinucleotide
Comments/Corrections

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