| Metabotyping of Long-Lived Mice using 1H NMR Spectroscopy. | |
| | |
MedLine Citation:
|
PMID: 22225495 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Significant advances in understanding aging have been achieved through studying model organisms with extended healthy lifespan. Employing 1H-NMR spectroscopy we characterized the plasma metabolic phenotype (metabotype) of three long-lived murine models: 30% dietary restricted (DR), insulin receptor substrate 1 null (Irs1-/-) and Ames dwarf (Prop1df/df). A panel of metabolic differences were generated for each model relative to their controls and subsequently the three long-lived models were compared to one another. Concentrations of mobile very low density lipoproteins, trimethylamine, and choline were significantly decreased in the plasma of all three models. Metabolites including glucose, choline, glycerophosphocholine and various lipids were significantly reduced; while acetoacetate, D-3-hydroxybutyrate and trimethylamine-N-oxide levels increased in DR compared to ad libitum controls. Plasma lipids and glycerophosphocholine were also decreased in Irs1-/- mice compared to controls, as were methionine and citrate. In contrast, high density lipoproteins and glycerophosphocholine were increased in Ames dwarf mice, as were methionine and citrate. Pairwise comparisons indicated differences existed between the metabotypes of the long-lived mice. Irs1-/- mice, for example, had elevated glucose, acetate, acetone and creatine, but lower methionine relative to DR mice and Ames dwarfs. Our study identified several potential candidate biomarkers directionally altered across all three models that may be predictive of longevity but also identified differences in the metabolic signatures. This comparative approach suggests that the metabolic networks underlying lifespan extension may not be exactly the same for each model of longevity and is consistent with multi-factorial control of the aging process. |
| | |
Authors:
|
Anisha Wijeyesekera; Colin Selman; Richard Hadlow Barton; Elaine Holmes; Dominic Withers; Jeremy Kirk Nicholson |
Related Documents
:
|
16529725 - P21cip1/waf1 regulates radial axon growth and enhances motor functional recovery in the... 8818975 - Myelination and axonal regeneration in the central nervous system of mice deficient in ... 19535585 - Versican v2 assembles the extracellular matrix surrounding the nodes of ranvier in the ... 17326765 - Defective peripheral nerve myelination and neuromuscular junction formation in fukutin-... 11404285 - Metabolic responses to leptin in obese db/db mice are strain dependent. 14637235 - Importance of fighting in the immune effects of social defeat. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-6 |
Journal Detail:
|
Title: Journal of proteome research Volume: - ISSN: 1535-3907 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Distribution and persistence of topical clotrimazole after sinus infusion in normal canine cadavers.
Next Document: Peptides Derived from Atlantic Salmon Skin Gelatin as Dipeptidyl-Peptidase IV Inhibitors.