Document Detail


Metabonomic and microbiological analysis of the dynamic effect of vancomycin-induced gut microbiota modification in the mouse.
MedLine Citation:
PMID:  18698804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of the antibiotic vancomycin (2 x 100 mg/kg/day) on the gut microbiota of female mice (outbred NMRI strain) were studied, in order to assess the relative contribution of the gut microbiome to host metabolism. The host's metabolic phenotype was characterized using (1)H NMR spectroscopy of urine and fecal extract samples. Time-course changes in the gut microbiotal community after administration of vancomycin were monitored using 16S rRNA gene PCR and denaturing gradient gel electrophoresis (PCR-DGGE) analysis and showed a strong effect on several species, mostly within the Firmicutes. Vancomycin treatment was associated with fecal excretion of uracil, amino acids and short chain fatty acids (SCFAs), highlighting the contribution of the gut microbiota to the production and metabolism of these dietary compounds. Clear differences in gut microbial communities between control and antibiotic-treated mice were observed in the current study. Reduced urinary excretion of gut microbial co-metabolites phenylacetylglycine and hippurate was also observed. Regression of urinary hippurate and phenylacetylglycine concentrations against the fecal metabolite profile showed a strong association between these urinary metabolites and a wide range of fecal metabolites, including amino acids and SCFAs. Fecal choline was inversely correlated with urinary hippurate. Metabolic profiling, coupled with the metagenomic study of this antibiotic model, illustrates the close inter-relationship between the host and microbial "metabotypes", and will provide a basis for further experiments probing the understanding of the microbial-mammalian metabolic axis.
Authors:
Ivan K S Yap; Jia V Li; Jasmina Saric; Francois-Pierre Martin; Huw Davies; Yulan Wang; Ian D Wilson; Jeremy K Nicholson; Jürg Utzinger; Julian R Marchesi; Elaine Holmes
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-08-13
Journal Detail:
Title:  Journal of proteome research     Volume:  7     ISSN:  1535-3893     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-08     Completed Date:  2008-11-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3718-28     Citation Subset:  IM    
Affiliation:
Department of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College London, Sir Alexander Fleming Building, South Kensington Campus, London SW7 2AZ, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Bacterial Agents / pharmacology*
Base Sequence
DNA Primers
Electrophoresis, Polyacrylamide Gel
Feces / chemistry
Female
Glycine / analogs & derivatives,  urine
Hippurates / urine
Intestines / microbiology*
Mice
Nuclear Magnetic Resonance, Biomolecular
Polymerase Chain Reaction
RNA, Ribosomal, 16S / genetics
Urine
Vancomycin / pharmacology*
Grant Support
ID/Acronym/Agency:
1-R01-HL084228-01A1/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/DNA Primers; 0/Hippurates; 0/RNA, Ribosomal, 16S; 1404-90-6/Vancomycin; 495-69-2/hippuric acid; 500-98-1/phenylacetylglycine; 56-40-6/Glycine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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