Document Detail

Metabolomics provide new insight on the metabolism of dietary phytochemicals in rats.
MedLine Citation:
PMID:  18567748     Owner:  NLM     Status:  MEDLINE    
Foods of plant origin contain a large number of phytochemicals that may positively affect health. Phytochemicals are largely excreted in urine as metabolites that are formed in host tissues or by the microbiota and constitute a great proportion of the urinary metabolome. The latter can be characterized by a metabolomics approach. In this work, we compared the metabolism of lignins to that of the structurally related ferulic acid (FA) and sinapic acid (SA). Five groups of rats (n = 5) were fed for 2 d a purified diet alone [control (C)] or supplemented with lignin-enriched wheat bran (3% of the diet, wt:wt), poplar wood lignins (0.42%), FA (0.42%), or SA (0.42%). The metabolomes of urine samples collected after 1 and 2 d of supplementation were analyzed by high-resolution MS (liquid chromatography/quadrupole time-of-flight). Comparing metabolic fingerprints by gathering semiquantitative information on several hundreds of metabolites and using multivariate statistical analysis (partial least squares for discriminant analysis) showed the similarity between both lignin-supplemented and C groups and confirmed that lignins are largely inert and not absorbed in the body. One the other hand, metabolic fingerprints of the 2 phenolic acid-supplemented groups were clearly distinct from the C group. Differences between the groups were mainly from nonmetabolized FA and SA and metabolites excreted in urine. Thirteen of them were identified as sulfate esters and glucuronide and glycine conjugates of the same phenolic acids, and of dihydrosinapic, vanillic, and benzoic acids. This study shows that metabolomics allows the identification of new metabolites of phytochemicals and can be used to distinguish individuals fed different phytochemical-containing foods.
Anthony Fardet; Rafael Llorach; Alexina Orsoni; Jean-François Martin; Estelle Pujos-Guillot; Catherine Lapierre; Augustin Scalbert
Related Documents :
8149948 - Biotransformation of pethidine: a comparative study of 24 h urine in three ethnic groups.
1793738 - Identification of nac-hcpc and nac-beta-cec, and qualitative analyses of sulphur amino ...
6629508 - Hippuric acid and o-cresol in the urine of workers exposed to toluene.
9746238 - Micellar electrokinetic chromatography for the determination of urinary desmosine and i...
23285238 - Rubisco evolution in c(4) eudicots: an analysis of amaranthaceae sensu lato.
7876858 - A missense point mutation (ser515phe) in the adrenoleukodystrophy gene in a family with...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of nutrition     Volume:  138     ISSN:  1541-6100     ISO Abbreviation:  J. Nutr.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-23     Completed Date:  2008-07-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1282-7     Citation Subset:  IM    
Unité de Nutrition Humaine, Institut National de la Recherche Agronomique, Centre de Recherche de Clermont-Ferrand/Theix, F-63122 St-Genès-Champanelle, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Coumaric Acids / metabolism*,  urine
Flavonoids / metabolism,  urine
Lignin / metabolism*,  urine
Phenols / metabolism,  urine
Plant Extracts / chemistry,  metabolism,  urine
Rats, Wistar
Reg. No./Substance:
0/Coumaric Acids; 0/Flavonoids; 0/Phenols; 0/Plant Extracts; 0/polyphenols; 1135-24-6/ferulic acid; 530-59-6/sinapinic acid; 9005-53-2/Lignin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The citrus flavonoids hesperetin and nobiletin differentially regulate low density lipoprotein recep...
Next Document:  A meal enriched with soy isoflavones increases nitric oxide-mediated vasodilation in healthy postmen...