Document Detail


Metabolomics as an extension of proteomic analysis: study of acute kidney injury.
MedLine Citation:
PMID:  18061843     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although proteomics studies the global expression of proteins, metabolomics characterizes and quantifies their end products: the metabolites, produced by an organism under a certain set of conditions. From this perspective it is apparent that proteomics and metabolomics are complementary and when joined allow a fuller appreciation of an organism's phenotype. Our studies using (1)H-nuclear magnetic resonance spectroscopic analysis showed the presence of glucose, amino acids, and trichloroacetic acid cycle metabolites in the urine after 48 hours of cisplatin administration. These metabolic alterations precede changes in serum creatinine. Biochemical studies confirmed the presence of glucosuria, but also showed the accumulation of nonesterified fatty acids, and triglycerides in serum, urine, and kidney tissue, despite increased levels of plasma insulin. These metabolic alterations were ameliorated by the use of fibrates. We propose that the injury-induced metabolic profile may be used as a biomarker of cisplatin-induced nephrotoxicity. These studies serve to illustrate that metabolomic studies add insight into pathophysiology not provided by proteomic analysis alone.
Authors:
Didier Portilla; Laura Schnackenberg; Richard D Beger
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Review    
Journal Detail:
Title:  Seminars in nephrology     Volume:  27     ISSN:  0270-9295     ISO Abbreviation:  Semin. Nephrol.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-02-15     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  8110298     Medline TA:  Semin Nephrol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  609-20     Citation Subset:  IM    
Affiliation:
Department of Nephrology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA. dportilla1@uams.edu
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MeSH Terms
Descriptor/Qualifier:
Acute Kidney Injury / chemically induced,  metabolism*
Cisplatin / toxicity
Fatty Acids / analysis
Glucose / metabolism
Humans
Hyperglycemia / chemically induced,  prevention & control
Insulin / blood
Kidney / metabolism
Magnetic Resonance Spectroscopy
PPAR alpha / physiology
Proteins / analysis*
Proteomics / methods*
Triglycerides / metabolism
Grant Support
ID/Acronym/Agency:
R01 DK075976/DK/NIDDK NIH HHS; R01 DK075976/DK/NIDDK NIH HHS; R01 DK075976-01A1/DK/NIDDK NIH HHS; R01 DK075976-02/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Insulin; 0/PPAR alpha; 0/Proteins; 0/Triglycerides; 15663-27-1/Cisplatin; 50-99-7/Glucose
Comments/Corrections

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