Document Detail

Metabolomics of aerobic metabolism in mice selected for increased maximal metabolic rate.
MedLine Citation:
PMID:  21982590     Owner:  NLM     Status:  MEDLINE    
Maximal aerobic metabolic rate (MMR) is an important physiological and ecological variable that sets an upper limit to sustained, vigorous activity. How the oxygen cascade from the external environment to the mitochondria may affect MMR has been the subject of much interest, but little is known about the metabolic profiles that underpin variation in MMR. We tested how seven generations of artificial selection for high mass-independent MMR affected metabolite profiles of two skeletal muscles (gastrocnemius and plantaris) and the liver. MMR was 12.3% higher in mass selected for high MMR than in controls. Basal metabolic rate was 3.5% higher in selected mice than in controls. Artificial selection did not lead to detectable changes in the metabolic profiles from plantaris muscle, but in the liver amino acids and tricarboxylic acid cycle (TCA cycle) metabolites were lower in high-MMR mice than in controls. In gastrocnemius, amino acids and TCA cycle metabolites were higher in high-MMR mice than in controls, indicating elevated amino acid and energy metabolism. Moreover, in gastrocnemius free fatty acids and triacylglycerol fatty acids were lower in high-MMR mice than in controls. Because selection for high MMR was associated with changes in the resting metabolic profile of both liver and gastrocnemius, the result suggests a possible mechanistic link between resting metabolism and MMR. In addition, it is well established that diet and exercise affect the composition of fatty acids in muscle. The differences that we found between control lines and lines selected for high MMR demonstrate that the composition of fatty acids in muscle is also affected by genetic factors.
Bernard Wone; Edward R Donovan; Jack P Hayes
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-09-16
Journal Detail:
Title:  Comparative biochemistry and physiology. Part D, Genomics & proteomics     Volume:  6     ISSN:  1878-0407     ISO Abbreviation:  Comp. Biochem. Physiol. Part D Genomics Proteomics     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-07     Completed Date:  2012-02-27     Revised Date:  2014-07-04    
Medline Journal Info:
Nlm Unique ID:  101270611     Medline TA:  Comp Biochem Physiol Part D Genomics Proteomics     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  399-405     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
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MeSH Terms
Basal Metabolism*
Fatty Acids / metabolism*
Gas Chromatography-Mass Spectrometry
Liver / metabolism
Mice / metabolism*
Muscle, Skeletal / metabolism
Grant Support
Reg. No./Substance:
0/Fatty Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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