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Metabolomic study of a diagnostic model for the metabolites of stool fat.
MedLine Citation:
PMID:  23354344     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Background/Aims: Metabolomics is a powerful tool for measuring low-molecular-weight metabolites in an organism at a specified time under specific environmental conditions. The aim of this study was to determine the usefulness of metabolomics in identifying the metabolites in stool-fat-positive specimens, and to establish whether the results could be used to predict the long-term prognosis.Methods: Fecal specimens were collected from 52 subjects with bowel habit change. The subjects were accessed using Rome III questionnaires and Bristol stool scale form, and followed after three years. The feces samples were centrifuged and the resulting extracts reconstituted for liquid chromatography/mass spectrometry analysis. The datasets were autoscaled, log-transformed, and mean-centered in a column-wise fashion prior to principal-components analysis and partial least-squares-discrimination analysis modeling.Results: Fecal samples from 10 of the 52 patients gave a positive stool-fat result of 30-100 mm; those of the remaining 42 contained neither fatty acids nor neutral fats. The peak intensities of lithocholic acid (p=0.001), lysophosphatidyl ethanolamine (lysoPE) 16:0 (p=0.015), and lysoPE 18:1/0:0 (p=0.014) were correlated with the size of the fatty acid. Subjects with positive stool-fat result showed higher score in Bristol stool scale form than those with negative stool-fat result at initial (p=0.040) and after three years (p=0.012).Conclusions: The metabolomic assay of stool fatty acid revealed mainly lysoPEs and lithocholic acid. The size of the fatty acid was correlated with higher concentrations of lysoPEs and lithocholic acid in stool-fat-test-positive specimens and related to loose stool even after three years of follow-up period. (Korean J Gastroenterol 2013;61:9-16).
Authors:
Choong Hwan Lee; Jiyoung Kim; Su Young Ahn; Sun Young Lee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi     Volume:  61     ISSN:  1598-9992     ISO Abbreviation:  Korean J Gastroenterol     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101189416     Medline TA:  Korean J Gastroenterol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  9-16     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Konkuk University School of Medicine, 120-1 Neungdong-ro, Gwangjin-gu, Seoul 143-729, Korea.
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