Document Detail

Metabolomic pattern of childhood neuroblastoma obtained by ¹H-high-resolution magic angle spinning (HRMAS) NMR spectroscopy.
MedLine Citation:
PMID:  20949594     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The aim of this preliminary study is to characterize by ¹H high-resolution magic angle spinning NMR spectroscopy (HRMAS) the metabolic content of intact biopsy samples obtained from 12 patients suffering from neuroblastoma (NB).
PROCEDURE: The biochemical NB profile was first compared to normal adrenal medulla. In a second step, the relationship between the tumor metabolic profile and the patients' clinical data was investigated.
RESULTS: A higher level of creatine, glutamine/glutamate, acetate and glycine characterized NB biopsies while healthy adrenal medulla tissue contained adrenaline and a larger amount of ascorbic acid. Adrenaline, which was undetectable in NB spectra, represented the metabolic signature of normal adrenal medulla. NB from patients younger than 12 months contained a higher level of acetate and lysine. Conversely, higher amounts of glutathione, glutamate, myo-inositol, glycine, serine and ascorbic acid were detected in NB samples belonging to younger children. Glutamine/glutamate, aspartate, creatine, glycine were characteristic of stage I-II NB. Acetate and creatine were characteristic of stage IV NB. Finally, a relatively higher amount of aspartate, succinate, and glutathione was detected in patients alive without active disease after a mean follow-up of 7 years whereas a higher concentration of acetate and taurine was characteristic of patients with worse prognosis.
CONCLUSIONS: Our preliminary results suggest the existence of a complex metabolic reality in NB, probably representative of tumor behavior. However, the real impact of these promising results should be assessed by long-term prospective studies on a larger cohort of patients.
Alessio Imperiale; Karim Elbayed; François-Marie Moussallieh; Agnès Neuville; Martial Piotto; Jean-Pierre Bellocq; Patrick Lutz; Izzie-Jacques Namer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-14
Journal Detail:
Title:  Pediatric blood & cancer     Volume:  56     ISSN:  1545-5017     ISO Abbreviation:  Pediatr Blood Cancer     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-11-25     Completed Date:  2010-12-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101186624     Medline TA:  Pediatr Blood Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  24-34     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Wiley-Liss, Inc.
Biophysics and Nuclear Medicine Department, University Hospitals of Strasbourg, Strasbourg, France.
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MeSH Terms
Adrenal Medulla / metabolism
Child, Preschool
Infant, Newborn
Magnetic Resonance Spectroscopy / methods*
Metabolomics / methods*
Neoplasm Staging
Neuroblastoma / metabolism*,  pathology

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