Document Detail


Metabolomic and mass isotopomer analysis of liver gluconeogenesis and citric acid cycle: II. Heterogeneity of metabolite labeling pattern.
MedLine Citation:
PMID:  18544526     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this second of two companion articles, we compare the mass isotopomer distribution of metabolites of liver gluconeogenesis and citric acid cycle labeled from NaH(13)CO(3) or dimethyl [1,4-(13)C(2)]succinate. The mass isotopomer distribution of intermediates reveals the reversibility of the isocitrate dehydrogenase + aconitase reactions, even in the absence of a source of alpha-ketoglutarate. In addition, in many cases, a number of labeling incompatibilities were found as follows: (i) glucose versus triose phosphates and phosphoenolpyruvate; (ii) differences in the labeling ratios C-4/C-3 of glucose versus (glyceraldehyde 3-phosphate)/(dihydroxyacetone phosphate); and (iii) labeling of citric acid cycle intermediates in tissue versus effluent perfusate. Overall, our data show that gluconeogenic and citric acid cycle intermediates cannot be considered as sets of homogeneously labeled pools. This probably results from the zonation of hepatic metabolism and, in some cases, from differences in the labeling pattern of mitochondrial versus extramitochondrial metabolites. Our data have implications for the use of labeling patterns for the calculation of metabolic rates or fractional syntheses in liver, as well as for modeling liver intermediary metabolism.
Authors:
Lili Yang; Takhar Kasumov; Rajan S Kombu; Shu-Han Zhu; Andrea V Cendrowski; France David; Vernon E Anderson; Joanne K Kelleher; Henri Brunengraber
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-06-10
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  283     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-04     Completed Date:  2008-09-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  21988-96     Citation Subset:  IM    
Affiliation:
Departments of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Isotopes / metabolism
Citric Acid / metabolism
Citric Acid Cycle / physiology*
Gluconeogenesis / physiology*
Glucose / metabolism
Isocitrate Dehydrogenase / metabolism
Isotope Labeling
Keto Acids / metabolism
Ketoglutaric Acids / metabolism
Liver / metabolism*
Phosphoenolpyruvate / metabolism
Rats
Sugar Phosphates / metabolism
Grant Support
ID/Acronym/Agency:
R33DK070291/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Keto Acids; 0/Ketoglutaric Acids; 0/Sugar Phosphates; 50-99-7/Glucose; 73-89-2/Phosphoenolpyruvate; 77-92-9/Citric Acid; EC 1.1.1.41/Isocitrate Dehydrogenase
Comments/Corrections

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