Document Detail

Metabolomic investigation into variation of endogenous metabolites in professional athletes subject to strength-endurance training.
MedLine Citation:
PMID:  19036890     Owner:  NLM     Status:  MEDLINE    
Strength-endurance type of sport can lead to modification of human beings' physiological status. The present study aimed to investigate the alteration of metabolic phenotype or biochemical compositions in professional athletes induced by long-term training by means of a novel systematic tool, metabolomics. Resting venous blood samples of junior and senior male rowers were obtained before and after 1-wk and 2-wk training. Venous blood from healthy male volunteers as control was also sampled at rest. Endogenous metabolites in serum were profiled by GC/TOF-MS and multivariate statistical technique, i.e., principal component analysis (PCA), and partial least squares projection to latent structures and discriminant analysis (PLS-DA) were used to process the data. Significant metabolomic difference was observed between the professional athletes and control subjects. Long-term strength and endurance training induced distinct separation between athletes of different exercise seniority, and training stage-related trajectory of the two groups of athletes was clearly shown along with training time. However, most of these variations were not observed by common biochemical parameters, such as hemoglobin, testosterone, and creatine kinase. The identified metabolites contributing to the classification included alanine, lactate, beta-d-methylglucopyranoside, pyroglutamic acid, cysteine, glutamic acid, citric acid, free fatty acids, valine, glutamine, phenylalanine, tyrosine, and so on, which were involved in glucose metabolism, oxidative stress, energy metabolism, lipid metabolism, amino acid metabolism. These findings suggest that metabolomics is a promising and potential tool to profile serum of professional athletes, make a deep insight into physiological states, and clarify the disorders induced by strength-endurance physical exercise.
Bei Yan; Jiye A; Guangji Wang; Huali Lu; Xiaoping Huang; Yi Liu; Weibin Zha; Haiping Hao; Ying Zhang; Linsheng Liu; Shenghua Gu; Qing Huang; Yuanting Zheng; Jianguo Sun
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-26
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  106     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-06     Completed Date:  2009-03-12     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  531-8     Citation Subset:  IM    
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
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MeSH Terms
Adaptation, Physiological
Amino Acids / metabolism
Biological Markers / blood*
Case-Control Studies
Creatine Kinase / blood
Discriminant Analysis
Energy Metabolism
Gas Chromatography-Mass Spectrometry
Glucose / metabolism
Hemoglobins / metabolism
Least-Squares Analysis
Lipid Metabolism
Metabolomics* / methods
Muscle Contraction*
Muscle, Skeletal / metabolism*
Oxidative Stress
Physical Endurance*
Principal Component Analysis
Testosterone / blood
Time Factors
Young Adult
Reg. No./Substance:
0/Amino Acids; 0/Biological Markers; 0/Hemoglobins; 50-99-7/Glucose; 58-22-0/Testosterone; EC Kinase

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