| Metabolomic Response to Exercise Training in Lean and Diet-Induced Obese Mice. | |
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MedLine Citation:
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PMID: 21270351 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Exercise training is a common therapeutic approach known to antagonize the metabolic consequences of obesity. Aims of the present study were to examine i) whether short-term, moderate intensity exercise training alters basal metabolite profile and ii) if 10d of mild exercise training can correct obesity-induced shifts in metabolic spectra. Following weaning, male C57BL/6J littermates were randomly divided into two diet groups: low (LF) or high fat (HF). After 12wk of dietary manipulation, HF animals were obese and hyperglycemic compared to LF. Mice from each group were further divided into sedentary or exercise treatments. Exercise training consisted of wheel running exercise (2h/d, 10d, 5.64m/min). Following exercise training, animals were rested (36h) and fasted (6h) prior to serum collection. Samples were analyzed by high resolution-1D proton NMR. Fifty high- and medium-concentration metabolites were identified. Pattern recognition algorithms and multivariate modeling were used to identify and isolate significant metabolites changing in response to HF and exercise training. Findings show that while exercise can mitigate some of the abnormal patterns in metabolic spectra induced by HF feeding, they can not negate it. In fact, when the effects of diet and exercise are compared, diet was a stronger predictor and had the larger influence on metabolic profile. External validation of models showed diet could be correctly classified with an accuracy of 89%, while exercise training could be classified 73% of the time. Results demonstrate metabolomics to effectively characterize obesity-induced perturbations in metabolism and support the concept that exercise is beneficial for this condition. |
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Authors:
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Gavin Duggan; Dustin S Hittel; Christoph W Sensen; Aalim M Weljie; Hans J Vogel; Jane Shearer |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-26 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: - ISSN: 1522-1601 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-1-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1University of Calgary. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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