Document Detail

Metabolomic response to exercise training in lean and diet-induced obese mice.
MedLine Citation:
PMID:  21270351     Owner:  NLM     Status:  MEDLINE    
Exercise training is a common therapeutic approach known to antagonize the metabolic consequences of obesity. The aims of the present study were to examine 1) whether short-term, moderate-intensity exercise training alters the basal metabolite profile and 2) if 10 days of mild exercise training can correct obesity-induced shifts in metabolic spectra. After being weaned, male C57BL/6J littermates were randomly divided into two diet groups: low fat (LF) or high fat (HF). After 12 wk of dietary manipulation, HF animals were obese and hyperglycemic compared with LF animals. Mice from each group were further divided into sedentary or exercise treatments. Exercise training consisted of wheel running exercise (2 h/day, 10 days, 5.64 m/min). After exercise training, animals were rested (36 h) and fasted (6 h) before serum collection. Samples were analyzed by high-resolution one-dimensional proton NMR. Fifty high- and medium-concentration metabolites were identified. Pattern recognition algorithms and multivariate modeling were used to identify and isolate significant metabolites changing in response to HF and exercise training. The results showed that while exercise can mitigate some of the abnormal patterns in metabolic spectra induced by HF diet feeding, they cannot negate it. In fact, when the effects of diet and exercise were compared, diet was a stronger predictor and had the larger influence on the metabolic profile. External validation of models showed that diet could be correctly classified with an accuracy of 89%, whereas exercise training could be classified 73% of the time. The results demonstrate metabolomics to effectively characterize obesity-induced perturbations in metabolism and support the concept that exercise is beneficial for this condition.
Gavin E Duggan; Dustin S Hittel; Christoph W Sensen; Aalim M Weljie; Hans J Vogel; Jane Shearer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-01-26
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  110     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-12     Completed Date:  2011-09-30     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1311-8     Citation Subset:  IM    
Department of Biological Sciences, Bio-NMR Center, Faculty of Science, University of Calgary, Calgary, Alberta, Canada.
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MeSH Terms
Dietary Fats*
Mice, Inbred C57BL
Obesity / chemically induced*,  metabolism*
Physical Conditioning, Animal / methods*
Physical Exertion
Proteome / metabolism*
Thinness / physiopathology*
Grant Support
//Canadian Institutes of Health Research
Reg. No./Substance:
0/Dietary Fats; 0/Proteome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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