Document Detail


Metabolomic effects of xylitol and fluoride on plaque biofilm in vivo.
MedLine Citation:
PMID:  21940519     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dental caries is initiated by demineralization of the tooth surface through acid production from sugar by plaque biofilm. Fluoride and xylitol have been used worldwide as caries-preventive reagents, based on in vitro-proven inhibitory mechanisms on bacterial acid production. We attempted to confirm the inhibitory mechanisms of fluoride and xylitol in vivo by performing metabolome analysis on the central carbon metabolism in supragingival plaque using the combination of capillary electrophoresis and a time-of-flight mass spectrometer. Fluoride (225 and 900 ppm F(-)) inhibited lactate production from 10% glucose by 34% and 46%, respectively, along with the increase in 3-phosphoglycerate and the decrease in phosphoenolpyruvate in the EMP pathway in supragingival plaque. These results confirmed that fluoride inhibited bacterial enolase in the EMP pathway and subsequently repressed acid production in vivo. In contrast, 10% xylitol had no effect on acid production and the metabolome profile in supragingival plaque, although xylitol 5-phosphate was produced. These results suggest that xylitol is not an inhibitor of plaque acid production but rather a non-fermentative sugar alcohol. Metabolome analyses of plaque biofilm can be applied for monitoring the efficacy of dietary components and medicines for plaque biofilm, leading to the development of effective plaque control.
Authors:
N Takahashi; J Washio
Related Documents :
21257189 - Cationic substrates of soybean lipoxygenase-1.
25186189 - A comparison of fruit quality parameters of wild bilberry (vaccinium myrtillus l.) grow...
8417029 - Biochemical properties and fatty acid composition of mycobacterium haemophilum: study o...
21690099 - A systems biology approach sheds new light on escherichia coli acid resistance.
25159309 - Aromatic acid derivatives from the lateral roots of aconitum carmichaelii.
11560379 - Isolation and structural characterization of colistin components.
22908119 - Amino acids as the source for producing carbon nanodots: microwave assisted one-step sy...
994749 - Lipid accumulation cells derived from porcine aorta and grown under anaerobic conditions.
6884439 - Long-term observations on the effect of polyadenylic acid in mice of different ages.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-09-22
Journal Detail:
Title:  Journal of dental research     Volume:  90     ISSN:  1544-0591     ISO Abbreviation:  J. Dent. Res.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-15     Completed Date:  2012-01-10     Revised Date:  2014-07-03    
Medline Journal Info:
Nlm Unique ID:  0354343     Medline TA:  J Dent Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1463-8     Citation Subset:  D; IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Cariostatic Agents / metabolism*
Dental Plaque / metabolism*
Electrophoresis, Capillary
Female
Fluorides / metabolism*
Glycolysis*
Humans
Lactic Acid / metabolism
Male
Metabolome*
Pentosephosphates / metabolism
Phosphoenolpyruvate / metabolism
Phosphopyruvate Hydratase / antagonists & inhibitors
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Streptococcus mutans / metabolism
Xylitol / metabolism*
Young Adult
Chemical
Reg. No./Substance:
0/Cariostatic Agents; 0/Pentosephosphates; 33X04XA5AT/Lactic Acid; 64913-51-5/xylitol 5-phosphate; 73-89-2/Phosphoenolpyruvate; EC 4.2.1.11/Phosphopyruvate Hydratase; Q80VPU408O/Fluorides; VCQ006KQ1E/Xylitol
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Meta-analysis of dysphagia and aspiration pneumonia in frail elders.
Next Document:  Periodontitis predicts elevated C-reactive protein levels in chronic kidney disease.