| Metabolomic assessment of the effect of dietary cholesterol in the progressive development of fatty liver disease. | |
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MedLine Citation:
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PMID: 20402505 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nonalcoholic fatty liver disease is considered to be the hepatic manifestation of metabolic syndrome and is usually related to high-fat, high-cholesterol diets. With the rationale that the identification and quantification of metabolites in different metabolic pathways may facilitate the discovery of clinically accessible biomarkers, we report the use of (1)H NMR metabolomics for quantitative profiling of liver extracts from LDLr(-/-) mice, a well-documented mouse model of fatty liver disease. A total of 55 metabolites were identified, and multivariate analyses in a diet- and time-comparative strategy were performed. Dietary cholesterol increased the hepatic concentrations of cholesterol, triglycerides, and oleic acid but also decreased the [PUFA/MUFA] ratio as well as the relative amount of long-chain polyunsaturated fatty acids in the liver. This was also accompanied by variations of the hepatic concentration of taurine, glutathione, methionine, and carnitine. Heat-map correlation analyses demonstrated that hepatic inflammation and development of steatosis correlated with cholesterol and triglyceride NMR derived signals, respectively. We conclude that dietary cholesterol is a causal factor in the development of both liver steatosis and hepatic inflammation. |
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Authors:
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Maria Vinaixa; Miguel Angel Rodríguez; Anna Rull; Raúl Beltrán; Cinta Bladé; Jesús Brezmes; Nicolau Cañellas; Jorge Joven; Xavier Correig |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of proteome research Volume: 9 ISSN: 1535-3907 ISO Abbreviation: J. Proteome Res. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-07 Completed Date: 2010-08-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: United States |
Other Details:
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Languages: eng Pagination: 2527-38 Citation Subset: IM |
Affiliation:
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Metabolomics Platform, CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), IISPV, Universitat Rovira i Virgili, Avda. Països Catalans 26, 43007 Tarragona, Spain. maria.vinaixa@urv.cat |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Animals Cholesterol, Dietary / administration & dosage, metabolism* Cluster Analysis Disease Progression Fatty Liver / metabolism* Histocytochemistry Inflammation / metabolism Male Metabolome* Metabolomics / methods* Mice Mice, Inbred C57BL Mice, Transgenic Multivariate Analysis Nuclear Magnetic Resonance, Biomolecular Receptors, LDL / genetics, metabolism Solubility Statistics, Nonparametric |
| Chemical | |
Reg. No./Substance:
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0/Cholesterol, Dietary; 0/Receptors, LDL |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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