Document Detail


Metabolite and transcriptome analysis of Campylobacter jejuni in vitro growth reveals a stationary-phase physiological switch.
MedLine Citation:
PMID:  19118349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Campylobacter jejuni is a prevalent cause of food-borne diarrhoeal illness in humans. Understanding of the physiological and metabolic capabilities of the organism is limited. We report a detailed analysis of the C. jejuni growth cycle in batch culture. Combined transcriptomic, phenotypic and metabolic analysis demonstrates a highly dynamic 'stationary phase', characterized by a peak in motility, numerous gene expression changes and substrate switching, despite transcript changes that indicate a metabolic downshift upon the onset of stationary phase. Video tracking of bacterial motility identifies peak activity during stationary phase. Amino acid analysis of culture supernatants shows a preferential order of amino acid utilization. Proton NMR (1H-NMR) highlights an acetate switch mechanism whereby bacteria change from acetate excretion to acetate uptake, most probably in response to depletion of other substrates. Acetate production requires pta (Cj0688) and ackA (Cj0689), although the acs homologue (Cj1537c) is not required. Insertion mutants in Cj0688 and Cj0689 maintain viability less well during the stationary and decline phases of the growth cycle than wild-type C. jejuni, suggesting that these genes, and the acetate pathway, are important for survival.
Authors:
John A Wright; Andrew J Grant; Douglas Hurd; Marcus Harrison; Edward J Guccione; David J Kelly; Duncan J Maskell
Related Documents :
24421199 - Evaluation of bacterial contamination in a fed-batch alcoholic fermentation process.
24039489 - New record of karoowia saxeti (stizenb.) hale in south korea.
20869309 - Alterations in d-amino acid concentrations and microbial community structures during th...
18961459 - Thermometric titration of acids in pyridine.
19543929 - Ammonium acetate enhances solvent production by clostridium acetobutylicum ea 2018 usin...
9921829 - Extension of shelf life of whole and peeled shrimp with organic acid salts and bifidoba...
4067259 - Effect of acute administration of bile acids on fatty acid composition of biliary phosp...
1249059 - Alanine and glutamine synthesis and release from skeletal muscle. ii. the precursor rol...
14541779 - Bio-surfactants.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  155     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-01     Completed Date:  2009-03-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  80-94     Citation Subset:  IM    
Affiliation:
Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK. j.wright@eastman.ucl.ac.uk
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acetates / metabolism*
Bacterial Proteins / genetics,  metabolism*
Campylobacter jejuni / genetics,  growth & development*,  metabolism,  physiology*
Colony Count, Microbial
Culture Media
Gene Expression Profiling*
Gene Expression Regulation, Bacterial*
Magnetic Resonance Spectroscopy
Microscopy, Phase-Contrast
Microscopy, Video
Movement
Oligonucleotide Array Sequence Analysis / methods
Phenotype
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Acetates; 0/Bacterial Proteins; 0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Characterization of an NADH oxidase of the flavin-dependent disulfide reductase family from Methanoc...
Next Document:  Involvement of the oscA gene in the sulphur starvation response and in Cr(VI) resistance in Pseudomo...