Document Detail

Metabolite and transcriptome analysis of Campylobacter jejuni in vitro growth reveals a stationary-phase physiological switch.
MedLine Citation:
PMID:  19118349     Owner:  NLM     Status:  MEDLINE    
Campylobacter jejuni is a prevalent cause of food-borne diarrhoeal illness in humans. Understanding of the physiological and metabolic capabilities of the organism is limited. We report a detailed analysis of the C. jejuni growth cycle in batch culture. Combined transcriptomic, phenotypic and metabolic analysis demonstrates a highly dynamic 'stationary phase', characterized by a peak in motility, numerous gene expression changes and substrate switching, despite transcript changes that indicate a metabolic downshift upon the onset of stationary phase. Video tracking of bacterial motility identifies peak activity during stationary phase. Amino acid analysis of culture supernatants shows a preferential order of amino acid utilization. Proton NMR (1H-NMR) highlights an acetate switch mechanism whereby bacteria change from acetate excretion to acetate uptake, most probably in response to depletion of other substrates. Acetate production requires pta (Cj0688) and ackA (Cj0689), although the acs homologue (Cj1537c) is not required. Insertion mutants in Cj0688 and Cj0689 maintain viability less well during the stationary and decline phases of the growth cycle than wild-type C. jejuni, suggesting that these genes, and the acetate pathway, are important for survival.
John A Wright; Andrew J Grant; Douglas Hurd; Marcus Harrison; Edward J Guccione; David J Kelly; Duncan J Maskell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  155     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-01     Completed Date:  2009-03-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  80-94     Citation Subset:  IM    
Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK.
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MeSH Terms
Acetates / metabolism*
Bacterial Proteins / genetics,  metabolism*
Campylobacter jejuni / genetics,  growth & development*,  metabolism,  physiology*
Colony Count, Microbial
Culture Media
Gene Expression Profiling*
Gene Expression Regulation, Bacterial*
Magnetic Resonance Spectroscopy
Microscopy, Phase-Contrast
Microscopy, Video
Oligonucleotide Array Sequence Analysis / methods
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
//Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Acetates; 0/Bacterial Proteins; 0/Culture Media

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