| Metabolite profiling of two low phytic acid (lpa) rice mutants. | |
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MedLine Citation:
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PMID: 18052121 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two low phytic acid (lpa) rice mutant lines, Os-lpa-XS110-1 and Os-lpa-XS110-2, were grown together with their parent wild-type variety Xiushui 110 in four field trials. HPLC analysis of inositol phosphates in the seeds produced demonstrated that compared to the wild-type, the reduction in phytic acid content in Os-lpa-XS110-1 (-46%) was more pronounced than that in Os-lpa-XS110-2 (-23%). Lower inositol phosphates (InsP 3, InsP 4, InsP 5) were not detected in the mutants. The lpa mutants and the wild-type rice were subjected to comparative metabolite profiling by capillary gas chromatography. On average, 34% (Os-lpa-XS110-1) and 42% (Os-lpa-XS110-2) of the detected peaks were statistically significantly different between wild-type and mutants. However, only a few of these differences could be consistently observed for all field trials. Identification and quantification of the consistently different metabolites revealed that contents of myo-inositol and raffinose were increased in Os-lpa-XS110-1 but decreased in Os-lpa-XS110-2 compared to the wild-type. In addition, Os-lpa-XS110-1 exhibited increased levels of galactose and galactinol. Consideration of these metabolic changes in light of the routes involved in the biosynthesis of phytic acid indicated a disturbance in the early biosynthetic pathway of phytic acid in Os-lpa-XS110-2 (similar to the lpa-1 type mutation in maize) and a mutation event affecting phosphorylation of myo-inositol in Os-lpa-XS110-1 (similar to the lpa-3-type mutation). |
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Authors:
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Thomas Frank; Bertrand Seumo Meuleye; Andreas Miller; Qing-Yao Shu; Karl-Heinz Engel |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-04 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 55 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-12-19 Completed Date: 2008-02-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 11011-9 Citation Subset: IM |
Affiliation:
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Lehrstuhl für Allgemeine Lebensmitteltechnologie, Technische Universität München, Freising-Weihenstephan, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Breeding Chromatography, High Pressure Liquid Inositol Phosphates / analysis Mutation* Oryza sativa / chemistry, genetics*, metabolism* Phytic Acid / analysis* Seeds / chemistry Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Inositol Phosphates; 83-86-3/Phytic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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