Document Detail


Metabolite profiling of tomato (Lycopersicon esculentum) using 1H NMR spectroscopy as a tool to detect potential unintended effects following a genetic modification.
MedLine Citation:
PMID:  12696919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The maize transcription factors LC and C1 were simultaneously overexpressed in tomato with the aim of producing lines with increased amounts of flavonols. The metabolite composition of these genetically modified tomatoes has been compared with that of azygous (nonmodified) controls grown side-by-side under the same conditions. It has been possible to observe metabolic changes in both types at different stages of maturity. (1)H NMR spectra showed that the levels of glutamic acid, fructose, and some nucleosides and nucleotides gradually increase from the immature to the ripe stage, whereas some amino acids such as valine and gamma-aminobutyric acid were present in higher amounts in unripe tomatoes. Apart from the significantly increased content of six main flavonoid glycosides (mainly kaempferol-3-O-rutinoside, with additional increases in kaempferol-3,7-di-O-glucoside (1), kaempferol-3-O-rutinoside-7-O-glucoside (2), kaempferol-3-O-glucoside, a dihydrokaempferol-O-hexoside (3), and naringenin-7-O-glucoside), the levels of at least 15 other metabolites were found to be different between the two types of red tomato. Among them were citric acid, sucrose, phenylalanine, and trigonelline. However, although statistically significant, these changes in mean values were relatively minor (less than 3-fold) and within the natural variation that would be observed in a field-grown crop. Nevertheless, this study clearly showed that NMR combined with chemometrics and univariate statistics can successfully trace even small differences in metabolite levels between plants and therefore represents a powerful tool to detect potential unintended effects in genetically modified crops.
Authors:
Gwénaëlle Le Gall; Ian J Colquhoun; Adrienne L Davis; Geoff J Collins; Martine E Verhoeyen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  51     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-16     Completed Date:  2003-07-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2447-56     Citation Subset:  IM    
Affiliation:
Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Carbohydrates / analysis
Glycosides / analysis
Lycopersicon esculentum / genetics,  growth & development,  metabolism*
Magnetic Resonance Spectroscopy / methods*
Nucleosides / analysis
Nucleotides / analysis
Plants, Genetically Modified / genetics,  growth & development,  metabolism*
Zea mays / genetics
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Glycosides; 0/Nucleosides; 0/Nucleotides
Comments/Corrections
Erratum In:
J Agric Food Chem. 2004 May 19;52(10):3210

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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