| Metabolite profiling of tomato (Lycopersicon esculentum) using 1H NMR spectroscopy as a tool to detect potential unintended effects following a genetic modification. | |
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MedLine Citation:
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PMID: 12696919 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The maize transcription factors LC and C1 were simultaneously overexpressed in tomato with the aim of producing lines with increased amounts of flavonols. The metabolite composition of these genetically modified tomatoes has been compared with that of azygous (nonmodified) controls grown side-by-side under the same conditions. It has been possible to observe metabolic changes in both types at different stages of maturity. (1)H NMR spectra showed that the levels of glutamic acid, fructose, and some nucleosides and nucleotides gradually increase from the immature to the ripe stage, whereas some amino acids such as valine and gamma-aminobutyric acid were present in higher amounts in unripe tomatoes. Apart from the significantly increased content of six main flavonoid glycosides (mainly kaempferol-3-O-rutinoside, with additional increases in kaempferol-3,7-di-O-glucoside (1), kaempferol-3-O-rutinoside-7-O-glucoside (2), kaempferol-3-O-glucoside, a dihydrokaempferol-O-hexoside (3), and naringenin-7-O-glucoside), the levels of at least 15 other metabolites were found to be different between the two types of red tomato. Among them were citric acid, sucrose, phenylalanine, and trigonelline. However, although statistically significant, these changes in mean values were relatively minor (less than 3-fold) and within the natural variation that would be observed in a field-grown crop. Nevertheless, this study clearly showed that NMR combined with chemometrics and univariate statistics can successfully trace even small differences in metabolite levels between plants and therefore represents a powerful tool to detect potential unintended effects in genetically modified crops. |
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Authors:
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Gwénaëlle Le Gall; Ian J Colquhoun; Adrienne L Davis; Geoff J Collins; Martine E Verhoeyen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 51 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2003 Apr |
Date Detail:
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Created Date: 2003-04-16 Completed Date: 2003-07-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 2447-56 Citation Subset: IM |
Affiliation:
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Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Carbohydrates / analysis Glycosides / analysis Lycopersicon esculentum / genetics, growth & development, metabolism* Magnetic Resonance Spectroscopy / methods* Nucleosides / analysis Nucleotides / analysis Plants, Genetically Modified / genetics, growth & development, metabolism* Zea mays / genetics |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbohydrates; 0/Glycosides; 0/Nucleosides; 0/Nucleotides |
| Comments/Corrections | |
Erratum In:
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J Agric Food Chem. 2004 May 19;52(10):3210 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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