| Metabolite profiling reveals distinct changes in carbon and nitrogen metabolism in phosphate-deficient barley plants (Hordeum vulgare L.). | |
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MedLine Citation:
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PMID: 18344526 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plants modify metabolic processes for adaptation to low phosphate (P) conditions. Whilst transcriptomic analyses show that P deficiency changes hundreds of genes related to various metabolic processes, there is limited information available for global metabolite changes of P-deficient plants, especially for cereals. As changes in metabolites are the ultimate 'readout' of changes in gene expression, we profiled polar metabolites from both shoots and roots of P-deficient barley (Hordeum vulgare) using gas chromatography-mass spectrometry (GC-MS). The results showed that mildly P-deficient plants accumulated di- and trisaccharides (sucrose, maltose, raffinose and 6-kestose), especially in shoots. Severe P deficiency increased the levels of metabolites related to ammonium metabolism in addition to di- and trisaccharides, but reduced the levels of phosphorylated intermediates (glucose-6-P, fructose-6-P, inositol-1-P and glycerol-3-P) and organic acids (alpha-ketoglutarate, succinate, fumarate and malate). The results revealed that P-deficient plants modify carbohydrate metabolism initially to reduce P consumption, and salvage P from small P-containing metabolites when P deficiency is severe, which consequently reduced levels of organic acids in the tricarboxylic acid (TCA) cycle. The extent of the effect of severe P deficiency on ammonium metabolism was also revealed by liquid chromatography-mass spectrometry (LC-MS) quantitative analysis of free amino acids. A sharp increase in the concentrations of glutamine and asparagine was observed in both shoots and roots of severely P-deficient plants. Based on these data, a strategy for improving the ability of cereals to adapt to low P environments is proposed that involves alteration in partitioning of carbohydrates into organic acids and amino acids to enable more efficient utilization of carbon in P-deficient plants. |
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Authors:
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Chun Y Huang; Ute Roessner; Ira Eickmeier; Yusuf Genc; Damien L Callahan; Neil Shirley; Peter Langridge; Antony Bacic |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-15 |
Journal Detail:
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Title: Plant & cell physiology Volume: 49 ISSN: 1471-9053 ISO Abbreviation: Plant Cell Physiol. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-05-15 Completed Date: 2008-07-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9430925 Medline TA: Plant Cell Physiol Country: Japan |
Other Details:
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Languages: eng Pagination: 691-703 Citation Subset: IM |
Affiliation:
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Australian Centre for Plant Functional Genomics, The University of Adelaide, Waite Campus, PMB1, Glen Osmond, South Australia, 5064, Australia. chunyuan.huang@adelaide.edu.au |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism Carbohydrate Metabolism / drug effects Carbon / metabolism* Gene Expression Regulation, Plant / drug effects Genes, Plant Hordeum / drug effects, genetics, growth & development, metabolism* Models, Biological Nitrogen / metabolism* Phosphates / deficiency*, pharmacology Plant Proteins / genetics, metabolism Plant Roots / drug effects, metabolism Plant Shoots / drug effects, metabolism Poaceae / drug effects, metabolism Quaternary Ammonium Compounds / pharmacology RNA, Messenger / genetics, metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Phosphates; 0/Plant Proteins; 0/Quaternary Ammonium Compounds; 0/RNA, Messenger; 7440-44-0/Carbon; 7727-37-9/Nitrogen |
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