Document Detail


Metabolite profiling reveals distinct changes in carbon and nitrogen metabolism in phosphate-deficient barley plants (Hordeum vulgare L.).
MedLine Citation:
PMID:  18344526     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plants modify metabolic processes for adaptation to low phosphate (P) conditions. Whilst transcriptomic analyses show that P deficiency changes hundreds of genes related to various metabolic processes, there is limited information available for global metabolite changes of P-deficient plants, especially for cereals. As changes in metabolites are the ultimate 'readout' of changes in gene expression, we profiled polar metabolites from both shoots and roots of P-deficient barley (Hordeum vulgare) using gas chromatography-mass spectrometry (GC-MS). The results showed that mildly P-deficient plants accumulated di- and trisaccharides (sucrose, maltose, raffinose and 6-kestose), especially in shoots. Severe P deficiency increased the levels of metabolites related to ammonium metabolism in addition to di- and trisaccharides, but reduced the levels of phosphorylated intermediates (glucose-6-P, fructose-6-P, inositol-1-P and glycerol-3-P) and organic acids (alpha-ketoglutarate, succinate, fumarate and malate). The results revealed that P-deficient plants modify carbohydrate metabolism initially to reduce P consumption, and salvage P from small P-containing metabolites when P deficiency is severe, which consequently reduced levels of organic acids in the tricarboxylic acid (TCA) cycle. The extent of the effect of severe P deficiency on ammonium metabolism was also revealed by liquid chromatography-mass spectrometry (LC-MS) quantitative analysis of free amino acids. A sharp increase in the concentrations of glutamine and asparagine was observed in both shoots and roots of severely P-deficient plants. Based on these data, a strategy for improving the ability of cereals to adapt to low P environments is proposed that involves alteration in partitioning of carbohydrates into organic acids and amino acids to enable more efficient utilization of carbon in P-deficient plants.
Authors:
Chun Y Huang; Ute Roessner; Ira Eickmeier; Yusuf Genc; Damien L Callahan; Neil Shirley; Peter Langridge; Antony Bacic
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-15
Journal Detail:
Title:  Plant & cell physiology     Volume:  49     ISSN:  1471-9053     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-05-15     Completed Date:  2008-07-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  691-703     Citation Subset:  IM    
Affiliation:
Australian Centre for Plant Functional Genomics, The University of Adelaide, Waite Campus, PMB1, Glen Osmond, South Australia, 5064, Australia. chunyuan.huang@adelaide.edu.au
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism
Carbohydrate Metabolism / drug effects
Carbon / metabolism*
Gene Expression Regulation, Plant / drug effects
Genes, Plant
Hordeum / drug effects,  genetics,  growth & development,  metabolism*
Models, Biological
Nitrogen / metabolism*
Phosphates / deficiency*,  pharmacology
Plant Proteins / genetics,  metabolism
Plant Roots / drug effects,  metabolism
Plant Shoots / drug effects,  metabolism
Poaceae / drug effects,  metabolism
Quaternary Ammonium Compounds / pharmacology
RNA, Messenger / genetics,  metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Phosphates; 0/Plant Proteins; 0/Quaternary Ammonium Compounds; 0/RNA, Messenger; 7440-44-0/Carbon; 7727-37-9/Nitrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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