Document Detail

Metabolite profiling of plasma and urine from rats with TNBS-induced acute colitis using UPLC-ESI-QTOF-MS-based metabonomics: A pilot study.
MedLine Citation:
PMID:  22520047     Owner:  NLM     Status:  Publisher    
The incidence of inflammatory bowel disease (IBD), a relapsing intestinal condition which precise etiology is still unclear, has continually increased in recent years. Metabolic profiling is an effective method with high sample throughput that can detect and identify potential biomarkers, and thus may be useful in investigating the pathogenesis of IBD. In this study, using a metabonomics approach, a pilot study based on ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) was firstly carried out to characterize the metabolic profile of plasma and urine samples of rats with experimental colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). Acquired metabolic profile data were processed by multivariate data analysis (MDA) for differentiating and screening of potential biomarkers. Five differential metabolites were identified in urine, namely two tryptophan metabolites [4-(2-Aminophenyl)-2,4-dioxobutanoic acid and 4,6-cihydroxyquinoline], two gut microbial metabolites (phenyl-acetylglycine and p-cresol glucuronide), and the bile acid 12α-hydroxy-3-oxocholadienic acid. Seven differential metabolites were identified in plasma, namely, three members of the bile acid/alcohol group (cholic acid, 12α-hydroxy-3-oxocholadienic acid and cholestane-3,7,12,24,25-pentol) and four lysophosphatidylcholines [LysoPCs : LysoPC (20:4), LysoPC (16:0), LysoPC (18:1) and LysoPC (18:0)]. These metabolites are associated with the damage of intestinal barrier function, microbiota homoeostasis, immune modulation and inflammatory response, and they play important roles in the pathogenesis of IBD. Our results positively support the application of metabonomic approach in pathophysiological mechanism study of IBD.
Xiaojun Zhang; Franky F K Choi; Yan Zhou; Feung Ping Leung; Shun Tan; Shuhai Lin; Hongxi Xu; Wei Jia; Joseph J Y Sung; Zongwei Cai; Zhaoxiang Bian
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-21
Journal Detail:
Title:  The FEBS journal     Volume:  -     ISSN:  1742-4658     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101229646     Medline TA:  FEBS J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Journal compilation © 2012 Federation of European Biochemical Societies.
School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China Chengdu Institute of Biology, The Chinese Academy of Sciences, Chengdu, Sichuan, China Department of Chemistry, Hong Kong Baptist University, Hong Kong SAR, China Shanghai University of Traditional Chinese Medicine, Pudong, Shanghai, China Department of Nutrition, University of North Carolina at Greensboro, North Carolina Research Campus, Kannapolis, NC 28081, USA Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
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