Document Detail

Metabolite profiling of hydroxycinnamate derivatives in plasma and urine after the ingestion of coffee by humans: identification of biomarkers of coffee consumption.
MedLine Citation:
PMID:  19460943     Owner:  NLM     Status:  MEDLINE    
Human subjects drank coffee containing 412 mumol of chlorogenic acids, and plasma and urine were collected 0 to 24 h after ingestion and were analyzed by high-performance liquid chromatography-mass spectrometry. Within 1 h, some of the components in the coffee reached nanomole peak plasma concentrations (C(max)), whereas chlorogenic acid metabolites, including caffeic acid-3-O-sulfate and ferulic acid-4-O-sulfate and sulfates of 3- and 4-caffeoylquinic acid lactones, had higher C(max) values. The short time to reach C(max) (T(max)) indicates absorption of these compounds in the small intestine. In contrast, dihydroferulic acid, its 4-O-sulfate, and dihydrocaffeic acid-3-O-sulfate exhibited much higher C(max) values (145-385 nM) with T(max) values in excess of 4 h, indicating absorption in the large intestine and the probable involvement of catabolism by colonic bacteria. These three compounds, along with ferulic acid-4-O-sulfate and dihydroferulic acid-4-O-glucuronide, were also major components to be excreted in urine (8.4-37.1 mumol) after coffee intake. Feruloylglycine, which is not detected in plasma, was also a major urinary component (20.7 mumol excreted). Other compounds, not accumulating in plasma but excreted in smaller quantities, included the 3-O-sulfate and 3-O-glucuronide of isoferulic acid, dihydro(iso)ferulic acid-3-O-glucuronide, and dihydrocaffeic acid-3-O-glucuronide. Overall, the 119.9 mumol excretion of the chlorogenic acid metabolites corresponded to 29.1% of intake, indicating that as well as being subject to extensive metabolism, chlorogenic acids in coffee are well absorbed. Pathways for the formation of the various metabolites within the body are proposed. Urinary dihydrocaffeic acid-3-O-sulfate and feruloylglycine are potentially very sensitive biomarkers for the consumption of relatively small amounts of coffee.
Angélique Stalmach; William Mullen; Denis Barron; Kenichi Uchida; Takao Yokota; Christophe Cavin; Heike Steiling; Gary Williamson; Alan Crozier
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-21
Journal Detail:
Title:  Drug metabolism and disposition: the biological fate of chemicals     Volume:  37     ISSN:  1521-009X     ISO Abbreviation:  Drug Metab. Dispos.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-20     Completed Date:  2009-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421550     Medline TA:  Drug Metab Dispos     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1749-58     Citation Subset:  IM    
Plant Products and Human Nutrition Group, Division of Environmental and Evolutionary Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK.
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MeSH Terms
Biological Markers / blood,  urine
Caffeic Acids / blood,  urine
Chromatography, High Pressure Liquid
Cinnamates / blood*,  pharmacokinetics,  urine*
Coffee / metabolism*
Coumaric Acids / blood*,  pharmacokinetics,  urine*
Glucuronic Acids / blood,  urine
Metabolomics* / methods
Spectrometry, Mass, Electrospray Ionization
Sulfates / blood,  urine
Reg. No./Substance:
0/Biological Markers; 0/Caffeic Acids; 0/Cinnamates; 0/Coffee; 0/Coumaric Acids; 0/Glucuronic Acids; 0/Sulfates; 1078-61-1/3,4-dihydroxyphenylpropionic acid; 1135-24-6/ferulic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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