Document Detail


Metabolite concentrations in follicular fluid may explain differences in fertility between heifers and lactating cows.
MedLine Citation:
PMID:  20385782     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There has been a marked decline in the fertility of dairy cows over the past decades, and metabolomic analysis offers a potential to investigate the underlying causes. Metabolite composition of the follicular fluid, which presents the intrafollicular environment, may be an important factor affecting oocyte maturation and subsequent early embryo development. The aim of the present study was to investigate the metabolic differences between follicular fluid from the dominant follicle of lactating cows and heifers using gas chromatography mass spectrometry (GC-MS)-based metabolomics. Follicular fluid and serum were collected from cows and heifers over three phases of follicle development: newly selected dominant follicles, preovulatory follicles prior to oestrus and post-LH surge follicles. Analysis of the fatty acids revealed that there were 24 fatty acids and 9 aqueous metabolites significantly different between cows and heifers. Of particular interest were the higher concentrations of saturated fatty acids (palmitic acid, P=0.001; stearic acid, P=0.005) in follicular fluid from cows and higher docosahexaenoic acid levels (P=0.022) in follicular fluid from heifers. Analysis of the metabolite composition of serum revealed that follicular fluid had a unique lipid composition. The higher concentrations of detrimental saturated fatty in cows will have a negative impact on oocyte maturation and early embryo development. Overall, the results suggest that the follicle microenvironment in cows potentially places their oocytes at a developmental disadvantage compared with heifers, and that this may contribute to well-characterised differences in fertility.
Authors:
K Bender; S Walsh; A C O Evans; T Fair; L Brennan
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-12
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  139     ISSN:  1741-7899     ISO Abbreviation:  Reproduction     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-20     Completed Date:  2010-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  1047-55     Citation Subset:  IM    
Affiliation:
UCD School of Agriculture, Food and Veterinary Medicine UCD Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle / metabolism*
Docosahexaenoic Acids / analysis
Embryonic Development / physiology
Female
Fertility / physiology*
Follicular Fluid / chemistry*
Gas Chromatography-Mass Spectrometry
Lactation / physiology*
Lipids / analysis,  blood
Metabolomics
Oocytes / growth & development
Ovarian Follicle / anatomy & histology,  metabolism
Palmitic Acid / analysis
Pregnancy
Stearic Acids / analysis
Chemical
Reg. No./Substance:
0/Lipids; 0/Stearic Acids; 25167-62-8/Docosahexaenoic Acids; 57-10-3/Palmitic Acid; 57-11-4/stearic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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