Document Detail


Metabolite-balancing techniques vs. 13C tracer experiments to determine metabolic fluxes in hybridoma cells.
MedLine Citation:
PMID:  10191398     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The estimation of intracellular fluxes of mammalian cells using only mass balances of the relevant metabolites is not possible because the set of linear equations defined by these mass balances is underdetermined. In order to quantify fluxes in cyclic pathways the mass balance equations can be complemented with several constraints: (1) the mass balances of co-metabolites, such as ATP or NAD(P)H, (2) linear objective functions, (3) flux data obtained by isotopic-tracer experiments. Here, these three methods are compared for the analysis of fluxes in the primary metabolism of continuously cultured hybridoma cells. The significance of different theoretical constraints and different objective functions is discussed after comparing their resulting flux distributions to the fluxes determined using 13CO2 and 13C-lactate measurements of 1 - 13C-glucose-fed hybridoma cells. Metabolic fluxes estimated using the objective functions "maximize ATP" and "maximize NADH" are relatively similar to the experimentally determined fluxes. This is consistent with the observation that cancer cells, such as hybridomas, are metabolically hyperactive, and produce ATP and NADH regardless of the need for these cofactors.
Authors:
H P Bonarius; B Timmerarends; C D de Gooijer; J Tramper
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  58     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:    1998 Apr 20-May 5
Date Detail:
Created Date:  1999-05-06     Completed Date:  1999-05-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  258-62     Citation Subset:  IM    
Copyright Information:
Copyright 1998 John Wiley & Sons, Inc.
Affiliation:
Department of Food Science, Food and Bioengineering Group, Wageningen, Agricultural University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.bona@novo.dk
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Animals
Carbon Isotopes
Cells, Cultured
Computational Biology
Hybridomas / metabolism*
Mass Spectrometry
Models, Biological*
NAD / metabolism
NADP / metabolism
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 53-59-8/NADP; 53-84-9/NAD; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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