Document Detail


Metabolism of the tricarboxylic acid cycle intermediates and related amino acids in ischemic guinea pig heart.
MedLine Citation:
PMID:  2893608     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The changes in the contents of the main tricarboxylic acid cycle intermediates and related amino acids under total ischemia and subsequent reperfusion of isolated guinea pig heart were studied. The decrease in ATP and phosphocreatine during 30 min ischemia was accompanied by alanine formation and approximately stoichiometric glutamate loss. The increase in malate in ischemic myocardium corresponded to the anaplerotic flux aspartate----oxaloacetate----malate. The succinate production was commensurable to alpha-ketoglutarate formation in the alanine aminotransferase reaction. The release of bulk amount of lactate, alanine and succinate into the myocardial effluent was observed during an early phase of the reperfusion using 1H NMR. In contrast to these metabolites, malate release was not observed in the reperfusion. By the 30th min of the reperfusion the decrease in lactate, alanine, malate and succinate tissue contents to the preischemic values was accompanied by the recovery of ATP and phosphocreatine. The results suggest that the formation and the release of succinate and alanine from the heart, complementary to that of lactate, reflect profound disturbances in energy metabolism.
Authors:
O Pisarenko; I Studneva; V Khlopkov
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biomedica biochimica acta     Volume:  46     ISSN:  0232-766X     ISO Abbreviation:  Biomed. Biochim. Acta     Publication Date:  1987  
Date Detail:
Created Date:  1988-03-15     Completed Date:  1988-03-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8304435     Medline TA:  Biomed Biochim Acta     Country:  GERMANY, EAST    
Other Details:
Languages:  eng     Pagination:  S568-71     Citation Subset:  IM    
Affiliation:
USSR Cardiology Research Center, Acad. Med. Sci. USSR, Moscow.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Alanine / metabolism
Amino Acids / metabolism*
Animals
Citric Acid Cycle*
Coronary Disease / metabolism*
Glutamates / metabolism
Glutamic Acid
Guinea Pigs
Myocardium / metabolism*
Perfusion
Phosphocreatine / metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Glutamates; 56-41-7/Alanine; 56-65-5/Adenosine Triphosphate; 56-86-0/Glutamic Acid; 67-07-2/Phosphocreatine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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