Document Detail


Metabolism of thrombospondin 2. Binding and degradation by 3t3 cells and glycosaminoglycan-variant Chinese hamster ovary cells.
MedLine Citation:
PMID:  8663244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thrombospondin 1 (TSP1) and thrombospondin 2 (TSP2) are members of the thrombospondin family that have a similar structural organization but somewhat different functional activities. Iodinated recombinant mouse TSP2 bound to NIH 3T3 cells and was internalized and degraded through a chloroquine-inhibitable pathway. TSP2 degradation was saturable, specific, and similar to the kinetics of degradation of TSP1. Human platelet TSP1, recombinant mouse TSP1, and recombinant mouse TSP2 cross-competed with one another for degradation by 3T3 cells. Degradation of TSP2 was less sensitive to inhibition by heparin than degradation of TSP1. This is in agreement with differences in heparin-binding affinity of the two TSPs. Degradation of TSP2 was slower in cultures of Chinese hamster ovary (CHO) cells lacking heparan sulfate proteoglycans than in wild type CHO cells or in cultures of 3T3 cells treated with heparitinase than in untreated 3T3 cells. Degradation of TSP2 was inhibited by antibodies against the low density lipoprotein receptor-related protein (LRP) or by the 39-kDa receptor-associated protein, a known antagonist of LRP. This study indicates that TSP2 and TSP1 are metabolized by a common internalization and degradation pathway involving heparan sulfate proteoglycan and LRP. Competition for this pathway is a possible mechanism whereby cells can control the levels and ratio of TSP1 and TSP2 in the extracellular milieu.
Authors:
H Chen; D K Strickland; D F Mosher
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  271     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-08-29     Completed Date:  1996-08-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  15993-9     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Wisconsin, Madison, 53706, USA.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Antibodies / pharmacology
Binding, Competitive
Blood Platelets / physiology*
CHO Cells
Cell Adhesion Molecules / metabolism
Chloroquine / pharmacology
Cricetinae
Fluorescent Antibody Technique, Indirect
Genetic Variation
Glycosaminoglycans / metabolism*
Heparan Sulfate Proteoglycans
Heparitin Sulfate / deficiency,  metabolism
Humans
Iodine Radioisotopes
Kinetics
LDL-Receptor Related Protein 1
Membrane Glycoproteins / metabolism*
Mice
Protein Binding
Proteoglycans / deficiency,  metabolism
Receptors, Immunologic / antagonists & inhibitors,  immunology,  physiology
Receptors, LDL / antagonists & inhibitors,  physiology
Recombinant Proteins / metabolism
Thrombospondins
Grant Support
ID/Acronym/Agency:
GM42581/GM/NIGMS NIH HHS; HL49111/HL/NHLBI NIH HHS; HL54462/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Cell Adhesion Molecules; 0/Glycosaminoglycans; 0/Heparan Sulfate Proteoglycans; 0/Iodine Radioisotopes; 0/LDL-Receptor Related Protein 1; 0/Membrane Glycoproteins; 0/Proteoglycans; 0/Receptors, Immunologic; 0/Receptors, LDL; 0/Recombinant Proteins; 0/Thrombospondins; 54-05-7/Chloroquine; 9050-30-0/Heparitin Sulfate

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