| Metabolism of stevioside and rebaudioside A from Stevia rebaudiana extracts by human microflora. | |
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MedLine Citation:
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PMID: 14558786 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stevia rebaudiana standardized extracts (SSEs) are used as natural sweeteners or dietary supplements in different countries for their content of stevioside or rebaudioside A. These compounds possess up to 250 times the sweetness intensity of sucrose, and they are noncaloric and noncariogenic sweeteners. The aim of this study was to investigate the in vitro transformation of stevioside and rebaudioside A after incubation with human microflora, the influence of these sweeteners on human microbial fecal community and which specific groups metabolize preferentially stevioside and rebaudioside A. The experiments were carried out under strict anaerobic conditions in batch cultures inoculated with mixed fecal bacteria from volunteers. The hydrolysis was monitored by HPLC coupled to photodiode array and mass spectrometric detectors. Isolated bacterial strains from fecal materials incubated in selective broths were added to stevioside and rebaudioside A. These sweeteners were completely hydrolyzed to their aglycon steviol in 10 and 24 h, respectively. Interestingly, the human intestinal microflora was not able to degrade steviol. Furthermore, stevioside and rebaudioside A did not significantly influence the composition of fecal cultures; among the selected intestinal groups, bacteroides were the most efficient in hydrolyzing Stevia sweeteners to steviol. |
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Authors:
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Claudio Gardana; Paolo Simonetti; Enrica Canzi; Raffaella Zanchi; Piergiorgio Pietta |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 51 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2003 Oct |
Date Detail:
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Created Date: 2003-10-15 Completed Date: 2003-12-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 6618-22 Citation Subset: IM |
Affiliation:
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Department of Food Science and Microbiology, Division of Human Nutrition, University of Milan, Via Celoria 2, 20133 Milan, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Bacteria / metabolism* Chromatography, High Pressure Liquid Diterpenes / metabolism* Diterpenes, Kaurane* Feces / microbiology* Female Glucosides / metabolism* Humans Male Mass Spectrometry Middle Aged Plant Extracts / chemistry* Stevia / chemistry* Sweetening Agents Terpenes / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Diterpenes; 0/Diterpenes, Kaurane; 0/Glucosides; 0/Plant Extracts; 0/Sweetening Agents; 0/Terpenes; 57817-89-7/stevioside; 58543-16-1/rebaudioside A |
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