| Metabolism of palmitic and docosahexaenoic acids in Reuber H35 hepatoma cells. | |
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MedLine Citation:
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PMID: 11011136 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this work, we have modified the fatty acid composition of Reuber H35 hepatoma cells by supplementation of the culture medium with a saturated (palmitic) or a polyunsaturated (docosahexaenoic) acid. These fatty acids were incorporated into total lipids and phospholipids of hepatoma cells. Palmitic acid readily increased the percentage of its monounsaturated derivative (16:1 n-7). When both fatty acids were supplemented at the same concentration, the percentage of docosahexaenoic acid in the total lipids and phospholipids of Reuber H35 cells increased more than that of palmitic acid. Although the levels of 16:0 increased, the addition of docosahexaenoic acid to the culture medium decreased the percentages of monoenoic acids. From our results, it can be concluded that palmitic and docosahexaenoic acids modify the fatty acid composition of Reuber H35 hepatoma cells. The profound changes induced by docosahexaenoic acid, especially those in the phospholipid fraction, may be of great interest given the main role of these components in the regulation of chemical and physical properties of biological membranes and/or membrane systems. |
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Authors:
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M Martínez-Cayuela; M C García-Pelayo; A Linares; E García-Peregrín |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biochemistry Volume: 128 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 2000 Oct |
Date Detail:
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Created Date: 2000-12-22 Completed Date: 2000-12-22 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 545-51 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, University of Granada, Granada, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Hepatocellular
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chemistry*,
metabolism* Cell Membrane / chemistry, metabolism Culture Media / chemistry, metabolism Docosahexaenoic Acids / analysis, metabolism* Fatty Acids / analysis, chemistry, metabolism Humans Lipid Metabolism Lipids / chemistry Palmitic Acid / analysis, metabolism* Phospholipids / chemistry, metabolism Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Fatty Acids; 0/Lipids; 0/Phospholipids; 25167-62-8/Docosahexaenoic Acids; 57-10-3/Palmitic Acid |
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