Document Detail


Metabolism of membrane phospholipids and its relation to a novel class of oligosaccharides in Escherichia coli.
MedLine Citation:
PMID:  4576016     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phosphatidylethanolamine is metabolically stable in exponentially growing cells of Escherichia coli, whereas phosphatidylglycerol and cardiolipin (diphosphatidylglycerol), labeled by growth in the presence of sn-[2-(3)H]glycero-3-(32)P, lose their radioactivity during subsequent growth on unlabeled medium. We have found that the loss of label from the lipid fraction is accompanied by its appearance in a novel type of cell constituent of E. coli, a closely related group of oligosaccharides, each containing glycerol and phosphoric acid in equimolar proportions, probably derived as intact glycerophosphate units from phosphatidylglycerol and/or cardiolipin. Glucose is the sole detectable sugar. The glycerophosphate-containing oligosaccharides may be fractionated by ion-exchange chromatography into several subfractions, some of which contain succinic acid in ester linkage. Although apparently derived from membrane phospholipids, the newly discovered substances contain no fatty acids, are soluble in water, and are insoluble in organic solvents such as chloroform. They comprise about 0.5-1% of the dry weight of growing cells.
Authors:
L M van Golde
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  70     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1973 May 
Date Detail:
Created Date:  1973-08-10     Completed Date:  1973-08-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1368-72     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cardiolipins / metabolism
Cell Membrane / metabolism
Chromatography
Chromatography, DEAE-Cellulose
Chromatography, Gel
Culture Media
Escherichia coli / growth & development,  metabolism*
Esters / isolation & purification
Fatty Acids / isolation & purification
Glucose / isolation & purification
Glycerol / metabolism
Glycerophosphates / metabolism
Hydrolysis
Oligosaccharides / analysis,  isolation & purification,  metabolism*
Phospholipids / metabolism*
Phosphorus Isotopes
Succinates / isolation & purification
Tritium
Chemical
Reg. No./Substance:
0/Cardiolipins; 0/Culture Media; 0/Esters; 0/Fatty Acids; 0/Glycerophosphates; 0/Oligosaccharides; 0/Phospholipids; 0/Phosphorus Isotopes; 0/Succinates; 10028-17-8/Tritium; 50-99-7/Glucose; 56-81-5/Glycerol
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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