| Metabolism of and inhibition by chlorobenzoates in Pseudomonas putida P111. | |
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MedLine Citation:
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PMID: 1781694 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pseudomonas putida P111 was isolated by enrichment culture on 2,5-dichlorobenzoate and was also able to grow on 2-chloro-, 3-chloro-, 4-chloro-, 2,3-dichloro-, 2,4-dichloro-, and 2,3,5-trichlorobenzoates. However, 3,5-dichlorobenzoate completely inhibited growth of P111 on all ortho-substituted benzoates that were tested. When 3,5-dichlorobenzoate was added as a cosubstrate with either 3- or 4-chlorobenzoate, cell yields and chloride release were greater than those observed from growth on either monochlorobenzoate alone. Moreover, resting cells of P111 grown on 4-chlorobenzoate released chloride from 3,5-dichlorobenzoate and produced no identifiable intermediate. In contrast, resting cells grown on 2,5-dichlorobenzoate metabolized 3,5-dichlorobenzoate without release of chloride and accumulated a degradation product, which was identified as 1-carboxy-1,2-dihydroxy-3,5-dichlorocyclohexadiene on the basis of gas chromatography-mass spectrometry confirmation of its two acid-hydrolyzed products, 3,5- and 2,4-dichlorophenol. Since 3,5-dichlorocatechol was rapidly metabolized by cells grown on 2,5-dichlorobenzoate, it is apparent that 1-carboxy-1,2-dihydroxy-3,5-dichlorocyclohexadiene is not further metabolized by these cells. Moreover, induction of a functional dihyrodiol dehydrogenase would not be required for growth of P111 on other ortho-chlorobenzoates since the corresponding chlorodihydrodiols produced from a 1,2-dioxygenase attack would spontaneously decompose to the corresponding catechols. In contrast, growth on 3-chloro-, 4-chloro-, or 3,5-dichlorobenzoate requires a functional dihydrodiol dehydrogenase, yet only the two monochlorobenzoates appear to induce for it. |
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Authors:
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B S Hernandez; F K Higson; R Kondrat; D D Focht |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 57 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 1991 Nov |
Date Detail:
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Created Date: 1992-03-12 Completed Date: 1992-03-12 Revised Date: 2010-09-09 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3361-6 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University of Panama, Republic of Panama. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biodegradation, Environmental Chlorobenzoates / chemistry, metabolism*, pharmacology Culture Media Environmental Pollutants / metabolism, pharmacology Pseudomonas putida / drug effects, growth & development*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Chlorobenzoates; 0/Culture Media; 0/Environmental Pollutants |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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