| Metabolism of human embryos following cryopreservation: implications for the safety and selection of embryos for transfer in clinical IVF. | |
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MedLine Citation:
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PMID: 17138583 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Cryopreservation of supernumerary embryos is routinely performed in human-assisted reproduction, providing a source of embryos which can be thawed for use in subsequent treatment cycles. However, the viability of cryopreserved embryos has traditionally relied on morphological assessment, which is a poor predictor of embryo health since freezing leads to a significant overall reduction in implantation potential, and its long-term efficacy is unknown. This study describes how the post-thaw metabolism of human embryos can be used to predict future development to the blastocyst stage. METHODS: HPLC was used to analyse the post-thaw amino acid metabolism of human embryos from day 2 to day 3 of development. RESULTS: It was possible to predict with 87% accuracy which frozen-thawed embryo would develop to the blastocyst stage. Developmentally competent embryos were more metabolically quiescent than their arresting counterparts. Amino acid turnover was also capable of distinguishing between the developmental potential of the best, Grade I embryos P < 0.05. CONCLUSIONS: The data suggests that cryopreservation in IVF is a safe procedure and that amino acid turnover can be used to select which cryopreserved embryo will develop to the blastocyst stage, irrespective of their post-thaw grade. |
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Authors:
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Paula J Stokes; Judith A Hawkhead; Richard K Fawthrop; Helen M Picton; Vinay Sharma; Henry J Leese; Franchesca D Houghton |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-11-30 |
Journal Detail:
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Title: Human reproduction (Oxford, England) Volume: 22 ISSN: 0268-1161 ISO Abbreviation: Hum. Reprod. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-19 Completed Date: 2007-05-04 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8701199 Medline TA: Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 829-35 Citation Subset: IM |
Affiliation:
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Department of Biology, University of York, York, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism* Chromatography, High Pressure Liquid Cryopreservation* Embryo Implantation Embryo Transfer* Embryo, Mammalian / metabolism*, physiology Female Fertilization in Vitro Humans |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids |
| Comments/Corrections | |
Erratum In:
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Hum Reprod. 2007 Apr;22(4):1196 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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