| Metabolism of cottonseed microsomal N-acylphosphatidylethanolamine. | |
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MedLine Citation:
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PMID: 7733669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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N-acylphosphatidylethanolamine (NAPE) was recently shown to be synthesized in vitro in cottonseed microsomes by the direct N-acylation of phosphatidylethanolamine (PE) with unesterfied fatty acids (K.D. Chapman and T. S. Moore, 1993, Plant Physiol. 102, 761-769). Here we examine the relationship of the synthesis and turnover of NAPE in cottonseed microsomes to the O-acylation of other membrane phospholipids. PE was N-acylated in a time-dependent manner with [1-14C]palmitic acid independent of exogenously supplied ATP. O-Acylation of PE and phosphatidylcholine (PC) with [1-14C]palmitic acid proceeded only in the presence of ATP. Further radiolabeling experiments with [1-14C]palmitoylCoA and phosphatidyl(N-[1-14C]-palmitoyl)ethanolamine indicated that O-acylation of phospholipids occurred via an acylCoA intermediate and not via an NAPE intermediate. [1-14C]palmitic acid was released from PC[1-14C-dipalmitoyl] in cottonseed microsomes in a Ca(2+)-dependent manner and this [14C]-FFA was incorporated into [14C]NAPE in a linear fashion. Cottonseed NAPE was selectively hydrolyzed to N-acylethanolamine (NAE) and N-acyl lysophosphatidylethanolamine (NAlysoPE) by Ca(2+)-independent, membrane-bound phospholipase D and A activities, respectively. NAlysoPE was not hydrolyzed to NAE, indicating that the phospholipase D that was active toward NAPE did not recognize NAlysoPE; instead NAlysoPE was converted to NAPE in the presence of Ca2+. Collectively, our results indicate that NAPE synthesis and the O-acylation of other phospholipids occur by two separate pathways and that microsomal NAPE is selectively turned over by membrane-bound phospholipase activities. A pathway for the metabolism of cottonseed NAPE is outlined. |
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Authors:
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K D Chapman; I Lin; A D DeSouza |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 318 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1995 Apr |
Date Detail:
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Created Date: 1995-05-26 Completed Date: 1995-05-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 401-7 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, University of North Texas, Denton 76203, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acylation Carbon Radioisotopes Chromatography, Thin Layer Gossypium / metabolism* Hydrolysis Kinetics Membrane Lipids / metabolism Microsomes / metabolism* Palmitic Acid Palmitic Acids / metabolism Phosphatidylethanolamines / metabolism* Phospholipids / metabolism Seeds / metabolism* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Membrane Lipids; 0/Palmitic Acids; 0/Phosphatidylethanolamines; 0/Phospholipids; 57-10-3/Palmitic Acid |
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