Document Detail


Metabolism of cottonseed microsomal N-acylphosphatidylethanolamine.
MedLine Citation:
PMID:  7733669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
N-acylphosphatidylethanolamine (NAPE) was recently shown to be synthesized in vitro in cottonseed microsomes by the direct N-acylation of phosphatidylethanolamine (PE) with unesterfied fatty acids (K.D. Chapman and T. S. Moore, 1993, Plant Physiol. 102, 761-769). Here we examine the relationship of the synthesis and turnover of NAPE in cottonseed microsomes to the O-acylation of other membrane phospholipids. PE was N-acylated in a time-dependent manner with [1-14C]palmitic acid independent of exogenously supplied ATP. O-Acylation of PE and phosphatidylcholine (PC) with [1-14C]palmitic acid proceeded only in the presence of ATP. Further radiolabeling experiments with [1-14C]palmitoylCoA and phosphatidyl(N-[1-14C]-palmitoyl)ethanolamine indicated that O-acylation of phospholipids occurred via an acylCoA intermediate and not via an NAPE intermediate. [1-14C]palmitic acid was released from PC[1-14C-dipalmitoyl] in cottonseed microsomes in a Ca(2+)-dependent manner and this [14C]-FFA was incorporated into [14C]NAPE in a linear fashion. Cottonseed NAPE was selectively hydrolyzed to N-acylethanolamine (NAE) and N-acyl lysophosphatidylethanolamine (NAlysoPE) by Ca(2+)-independent, membrane-bound phospholipase D and A activities, respectively. NAlysoPE was not hydrolyzed to NAE, indicating that the phospholipase D that was active toward NAPE did not recognize NAlysoPE; instead NAlysoPE was converted to NAPE in the presence of Ca2+. Collectively, our results indicate that NAPE synthesis and the O-acylation of other phospholipids occur by two separate pathways and that microsomal NAPE is selectively turned over by membrane-bound phospholipase activities. A pathway for the metabolism of cottonseed NAPE is outlined.
Authors:
K D Chapman; I Lin; A D DeSouza
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  318     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-05-26     Completed Date:  1995-05-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  401-7     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, University of North Texas, Denton 76203, USA.
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MeSH Terms
Descriptor/Qualifier:
Acylation
Carbon Radioisotopes
Chromatography, Thin Layer
Gossypium / metabolism*
Hydrolysis
Kinetics
Membrane Lipids / metabolism
Microsomes / metabolism*
Palmitic Acid
Palmitic Acids / metabolism
Phosphatidylethanolamines / metabolism*
Phospholipids / metabolism
Seeds / metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Membrane Lipids; 0/Palmitic Acids; 0/Phosphatidylethanolamines; 0/Phospholipids; 57-10-3/Palmitic Acid

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