Document Detail

Metabolism of cottonseed microsomal N-acylphosphatidylethanolamine.
MedLine Citation:
PMID:  7733669     Owner:  NLM     Status:  MEDLINE    
N-acylphosphatidylethanolamine (NAPE) was recently shown to be synthesized in vitro in cottonseed microsomes by the direct N-acylation of phosphatidylethanolamine (PE) with unesterfied fatty acids (K.D. Chapman and T. S. Moore, 1993, Plant Physiol. 102, 761-769). Here we examine the relationship of the synthesis and turnover of NAPE in cottonseed microsomes to the O-acylation of other membrane phospholipids. PE was N-acylated in a time-dependent manner with [1-14C]palmitic acid independent of exogenously supplied ATP. O-Acylation of PE and phosphatidylcholine (PC) with [1-14C]palmitic acid proceeded only in the presence of ATP. Further radiolabeling experiments with [1-14C]palmitoylCoA and phosphatidyl(N-[1-14C]-palmitoyl)ethanolamine indicated that O-acylation of phospholipids occurred via an acylCoA intermediate and not via an NAPE intermediate. [1-14C]palmitic acid was released from PC[1-14C-dipalmitoyl] in cottonseed microsomes in a Ca(2+)-dependent manner and this [14C]-FFA was incorporated into [14C]NAPE in a linear fashion. Cottonseed NAPE was selectively hydrolyzed to N-acylethanolamine (NAE) and N-acyl lysophosphatidylethanolamine (NAlysoPE) by Ca(2+)-independent, membrane-bound phospholipase D and A activities, respectively. NAlysoPE was not hydrolyzed to NAE, indicating that the phospholipase D that was active toward NAPE did not recognize NAlysoPE; instead NAlysoPE was converted to NAPE in the presence of Ca2+. Collectively, our results indicate that NAPE synthesis and the O-acylation of other phospholipids occur by two separate pathways and that microsomal NAPE is selectively turned over by membrane-bound phospholipase activities. A pathway for the metabolism of cottonseed NAPE is outlined.
K D Chapman; I Lin; A D DeSouza
Related Documents :
25492199 - Magnetic ph-responsive poly(methacrylic acid-co-acrylic acid)-co-polyvinylpyrrolidone m...
2358449 - Export of mitochondrially synthesized lysophosphatidic acid.
6707609 - The lipids of the rumen fungus piromonas communis.
9705349 - Human type 2 phosphatidic acid phosphohydrolases. substrate specificity of the type 2a,...
18195019 - Identification and characterization of a major liver lysophosphatidylcholine acyltransf...
8353529 - Dietary ethanol stimulates the activity of phosphatidylcholine-specific phospholipase d...
132679 - The effect of fractionated administration of locetamic acid (cholebrine) on gallbladder...
18967289 - Detection and spectrophotometric determination of edta with cobalt(ii) and phosphomolyb...
19371719 - The role of fatty acid unsaturation in minimizing biophysical changes on the structure ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  318     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-05-26     Completed Date:  1995-05-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  401-7     Citation Subset:  IM    
Department of Biological Sciences, University of North Texas, Denton 76203, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carbon Radioisotopes
Chromatography, Thin Layer
Gossypium / metabolism*
Membrane Lipids / metabolism
Microsomes / metabolism*
Palmitic Acid
Palmitic Acids / metabolism
Phosphatidylethanolamines / metabolism*
Phospholipids / metabolism
Seeds / metabolism*
Time Factors
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Membrane Lipids; 0/Palmitic Acids; 0/Phosphatidylethanolamines; 0/Phospholipids; 57-10-3/Palmitic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cytochrome and alternative respiratory pathways compete for electrons in the presence of pyruvate in...
Next Document:  Superoxide from glucose oxidase or from nitroblue tetrazolium?