| Metabolism of arachidonic acid by isolated rat hepatocytes, renal cells and by some rabbit tissues. Detection of vicinal diols by mass fragmentography. | |
| | |
MedLine Citation:
|
PMID: 6814498 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Purified cytochromes P-450 (LM2 and PB-B2) in a reconstituted system and epoxide hydrolase were recently found to metabolize arachidonic (eicosatetraenoic) acid to four vicinal dihydroxyeicosatrienoic acids. These metabolites were chemically synthetized from octadeuterated arachidonic acid and employed as internal standards for mass fragmentography. Isolated rat hepatocytes and renal cells were incubated with arachidonic acid (0.1 mM; 37 degrees C, 15 min) and, following extractive isolation and reversed-phase HPLC, formation of 11,12-dihydroxy-5,8,14-eicosatrienoic acid and 14,15-dihydroxy-5,8,11-eicosatrienoic acid was demonstrated by mass fragmentography using a capillary GC column. Furthermore, these diols were also detected in rabbit liver and renal cortex and they therefore appear to be formed endogenously. Formation of vicinal diols was also studied in cell free systems. Rabbit liver and renal cortical microsomes were incubated with NADPH (1 mM) and arachidonic acid (0.15 mM) for 15 min at 37 degree C and, besides 11,12-dihydroxy- and 14,15-dihydroxyeicosatrienoic acid, small amounts of 8,9-dihydroxy- and 5,6-dihydroxyeicosatrienoic acid could be detected by mass fragmentography. Renal as wall as hepatic monooxygenases can thus epoxidize each of the four double bonds of arachidonic acid. In contrast, rabbit lung microsomes and NADPH metabolized arachidonic acid mainly to prostaglandins and 19-hydroxy- and 20-hydroxyarachidonic acid, while only small amounts of 11,12-dihydroxyeicosatrienoic acid could be found. Monooxygenase metabolism of arachidonic acid by epoxidation might therefore be a significant pathway for the metabolism of this essential fatty acid in isolated rat renal cells and hepatocytes but presumably not in the lung. |
| | |
Authors:
|
E H Oliw; P Moldéus |
Related Documents
:
|
617808 - Metabolism of arachidonic acid in rabbit alveolar macrophages. 17121818 - Chemical determinants involved in anandamide-induced inhibition of t-type calcium chann... 9602108 - Arachidonic acid-sensitive a-currents and multiple kv4 transcripts are expressed in chi... 17405868 - Flufenamic acid bi-directionally modulates the transient outward k(+) current in rat ce... 11388848 - Direct mono-n-methylation of solid-supported amino acids: a useful application of the m... 1621808 - Substrate responses to acid-base alterations in dog renal proximal tubules. |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 721 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1982 Oct |
Date Detail:
|
Created Date: 1983-01-19 Completed Date: 1983-01-19 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 135-43 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arachidonic Acid Arachidonic Acids / metabolism* Chromatography, High Pressure Liquid Cytochrome P-450 Enzyme System / metabolism Gas Chromatography-Mass Spectrometry Kidney / metabolism* Liver / metabolism* Lung / metabolism Male Microsomes / metabolism Organ Specificity Rats Rats, Inbred Strains Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
|
0/Arachidonic Acids; 506-32-1/Arachidonic Acid; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Allantoinase from Pseudomonas aeruginosa. Purification, properties and immunochemical characterizati...
Next Document: Regulation and autoregulation by lexA protein.