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Metabolism of uronic acids in plant tissues: partial purification and properties of uronic Acid oxidase from citrus leaves.
MedLine Citation:
PMID:  16659133     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
A new enzyme, named uronic acid oxidase, was extracted and purified 67-fold by (NH(4))(2)SO(4) fractionation and CM-Sephadex column chromatography from ethylene-treated Shamouti orange (Citrus sinensis L. Osbeck) leaves. The enzyme catalyzes the oxidation of d-galacturonic acid and d-glucuronic acid to the corresponding hexaric acids in the presence of molecular oxygen with the production of H(2)O(2). The pH optimum for the oxidation of d-galacturonic acid and d-glucuronic acid is between 7 and 8. The enzyme is highly specific for d-galacturonic acid and d-glucuronic acid. It also oxidizes polygalacturonic acid. The apparent Michaelis constant values of the enzyme for d-galacturonic acid and d-glucuronic acid are 0.13 and 0.5 mm, respectively. The molecular weight of the enzyme, as determined by gel filtration, is about 98,000. The enzyme is inhibited by sodium hydrosulfite and other sulfites, indicating that it contains a flavin prosthetic group.
Authors:
J Riov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  55     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1975 Apr 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  602-6     Citation Subset:  -    
Affiliation:
Department of Horticulture, The Hebrew University of Jerusalem, Rehovot, Israel.
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