| Metabolism of uronic acids in plant tissues: partial purification and properties of uronic Acid oxidase from citrus leaves. | |
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MedLine Citation:
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PMID: 16659133 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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A new enzyme, named uronic acid oxidase, was extracted and purified 67-fold by (NH(4))(2)SO(4) fractionation and CM-Sephadex column chromatography from ethylene-treated Shamouti orange (Citrus sinensis L. Osbeck) leaves. The enzyme catalyzes the oxidation of d-galacturonic acid and d-glucuronic acid to the corresponding hexaric acids in the presence of molecular oxygen with the production of H(2)O(2). The pH optimum for the oxidation of d-galacturonic acid and d-glucuronic acid is between 7 and 8. The enzyme is highly specific for d-galacturonic acid and d-glucuronic acid. It also oxidizes polygalacturonic acid. The apparent Michaelis constant values of the enzyme for d-galacturonic acid and d-glucuronic acid are 0.13 and 0.5 mm, respectively. The molecular weight of the enzyme, as determined by gel filtration, is about 98,000. The enzyme is inhibited by sodium hydrosulfite and other sulfites, indicating that it contains a flavin prosthetic group. |
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Authors:
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J Riov |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Plant physiology Volume: 55 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 1975 Apr |
Date Detail:
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Created Date: 2010-06-29 Completed Date: 2010-06-29 Revised Date: 2010-09-15 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 602-6 Citation Subset: - |
Affiliation:
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Department of Horticulture, The Hebrew University of Jerusalem, Rehovot, Israel. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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