| Metabolism of Tanshinone IIA, Cryptotanshinone and Tanshinone I from Radix Salvia Miltiorrhiza in Zebrafish. | |
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MedLine Citation:
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PMID: 22810195 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The study aimed to investigate the potential of zebrafish in imitating mammal phase I metabolism of natural compounds. Three diterpenoid quinones from Radix Salvia miltiorrhiza, namely tanshinone IIA (TIIA), cryptotanshinone (Cry) and tanshinone I (TI) were selected as model compounds, and their metabolites mediated by zebrafish were characterized using a high-performance liquid chromatography coupled ion-trap mass spectrometry (HPLC/IT-MSn) method with electrospray ionization in positive mode. The separation was performed with a Zorbax C-18 column using a binary gradient elution of 0.05% formic acid acetonitrile/0.05% formic acid water. According to the MS spectra and after comparison with reference standards and literature reports, hydroxylation, dehydrogenation or D-ring hydrolysis metabolites of TIIA and Cry but not of TI were characterized, which coincided with those reported using regular <em>in vivo</em> or <em>in vitro</em> metabolic analysis methods, thus verifying that zebrafish can successfully imitate mammalian phase I metabolism which instills further confidence in using zebrafish as a novel and prospective metabolism model. |
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Authors:
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Yingjie Wei; Ping Li; Changmei Wang; Yunru Peng; Luan Shu; Xiaobin Jia; Wenquan Ma; Bing Wang |
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Publication Detail:
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Type: Journal Article Date: 2012-07-18 |
Journal Detail:
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Title: Molecules (Basel, Switzerland) Volume: 17 ISSN: 1420-3049 ISO Abbreviation: Molecules Publication Date: 2012 |
Date Detail:
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Created Date: 2012-07-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100964009 Medline TA: Molecules Country: Switzerland |
Other Details:
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Languages: eng Pagination: 8617-32 Citation Subset: IM |
Affiliation:
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Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, 100 Shizi Street, Nanjing 210028, China. liping2004@126.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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