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Metabolism of Oxidized High Density Lipoprotein-2(ox-HDL(2)) in Rat Hepatic Sinusoidal Cells.
MedLine Citation:
PMID:  12168034     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Incubation of rat hepatic sinusoidal cells with FITC-HDL(2), FITC-ox-HDL(2) and [(3)H]CE-HDL(2)(rHDL(2)), ox-rHDL(2) showed that binding of FITC-HDL(2) to the cells was competitive to ox-HDL(2), but not to HDL(2). The cell-endocytic fluorescence strength (FS) of FITC-HDL(2) and radioactivity of ox-rHDL(2) were 45.5% of that of FITC-HDL(2) and 61.4% of that of rHDL(2), respectively. Endocytic FS and radioactivity were mainly in TCA-precipitable and supernatant part, respectively. The cell-released FS and radioactivity were 67.7% and 10.9% of the cell-endocytic FS and the radioactivity, respectively, and both of them were mainly TCA-precipitable. These results suggest that: (1) There is probably an ox-HDL receptor on the surface of rat hepatic sinusoidal cells, which is different from HDL receptor. (2) The metabolic behaviour of ox-rHDL(2) in the cells is similar to HDL(2). Both of them do not take a lysosomal pathway. Apoproteins and CE components dissociate from endocytic lipoprotein in the cells. After the cells have taken up most of CE, the residual CE recombines with apolipoprotein to form a lipoprotein and is released from the cells by retroendocytosis. (3) Oxidative modification of HDL(2) weakens its ability to cholesterol reverse transport.
Authors:
An Cheng; Man-Ping Wu; Bin Lou; Pei-Fang Chen; Xiao-Qi Yang; Mei-Zhen Mei
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica     Volume:  30     ISSN:  0582-9879     ISO Abbreviation:  Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao     Publication Date:  1998  
Date Detail:
Created Date:  2002-Aug-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  20730160R     Medline TA:  Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  343-346     Citation Subset:  -    
Affiliation:
Department of Biochemistry, School of Pharmacy, Shanghai Medical University, Shanghai 200032, China. qqzhuang@shmu.edu.cn
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