| Metabolism of D- and L-[(13)C]alanine in rat liver detected by (1)H and (13)C NMR spectroscopy in vivo and in vitro. | |
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MedLine Citation:
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PMID: 10797635 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Alanine is the major amino acid utilized by the liver for gluconeogenesis under normal conditions. The metabolism of alanine in rat liver was investigated by means of (1)H and (13)C NMR spectroscopic studies in vivo and in vitro after infusion of L- and D-alanine labelled with (13)C at the carboxyl and methyl group into normal, fasted rats. Valuable information about different metabolic pathways of alanine in rat liver and their regulation under the conditions of gluconeogenesis were obtained. The enrichment of the alanine pool by the infusate was estimated to be 11% for L-alanine and 70% for D-alanine. After infusion of labelled D-alanines, the metabolic pathway via D-amino acid oxidase was observed. The labelled alanines entered the tricarboxylic acid cycle mainly via pyruvate carboxylase; the ratio of pyruvate dehydrogenase activity to that of pyruvate carboxylase is about 28%. The ratio of flux from phosphoenolpyruvate (PEP) through phosphoenolpyruvate kinase as compared with the flux from PEP to glucose was approximately 42%. From the labelling pattern of glucose it was concluded that the pentose phosphate cycle was active under the experimental conditions. |
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Authors:
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A Dölle |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: NMR in biomedicine Volume: 13 ISSN: 0952-3480 ISO Abbreviation: NMR Biomed Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-05-31 Completed Date: 2000-05-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8915233 Medline TA: NMR Biomed Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 72-81 Citation Subset: IM |
Copyright Information:
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Copyright 2000 John Wiley & Sons, Ltd. |
Affiliation:
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Abteilung Biophysikalische Chemie, Biozentrum der Universität Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland. doelle@rwth-aachen.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alanine
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metabolism* Amino Acids / metabolism* Animals Carbon Isotopes Gluconeogenesis Glucose / metabolism* Hydrogen Liver / metabolism* Magnetic Resonance Spectroscopy / methods Male Models, Biological Rats Rats, Sprague-Dawley Stereoisomerism |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbon Isotopes; 1333-74-0/Hydrogen; 50-99-7/Glucose; 56-41-7/Alanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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