| Metabolism of 5,6-epoxyretinoic acid in vivo: isolation of a major intestinal metabolite. | |
| | |
MedLine Citation:
|
PMID: 7082654 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The major metabolite in the small intestinal mucosa of vitamin A deficient rats dosed intrajugularly with 5,6-epoxy[3H]-retinoic acid has been identified as 5,6-epoxyretinoyl beta-glucuronide. The assignment was based on the metabolite's chemical, spectral, and chromatographic properties. Incubation of the metabolite with beta-glucuronidase released 5,6-epoxyretinoic acid. Incubation of 5,6-epoxyretinoic acid with rat liver microsomes in the presence of uridine-5'-diphospho-1 alpha-D-glucuronic acid produced the metabolite. 5,6-Epoxy[3H]retinoyl beta-glucuronide weas observed in the liver, small intestinal mucosa, and intestinal contents but not in kidney of vitamin a deficient rats. Its concentration was greatly diminished in liver and small intestinal mucosa, and it was not observed in kidney of vitamin A deficient rats dosed orally with retinoic acid for several days before administration of 5,6-epoxy[3H]retinoic acid. Generally, oral retinoic acid treatment accelerated 5,6-epoxyretinoic acid metabolism and enhanced accumulation of highly polar metabolites. Moreover, 5,6-epoxyretinoic acid metabolism was more rapid than that of retinoic acid and did not result in production of retinoic acid. |
| | |
Authors:
|
J L Napoli; H Khalil; A M McCormick |
Related Documents
:
|
15180384 - Effects of methoprene, its metabolites, and breakdown products on retinoid-activated pa... 11237754 - Streptococcus mutans lipoteichoic acid-induced apoptosis in cultured dental pulp cells ... 22440424 - Prediction of fat quality in pig carcasses by near-infrared spectroscopy. 11896294 - Prevention of vitamin a teratogenesis by phytol or phytanic acid results from reduced m... 11333844 - Amino acid kinetics in patients with sepsis. 1373864 - Study of unscheduled dna synthesis following exposure of human cells to arecoline and e... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biochemistry Volume: 21 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1982 Apr |
Date Detail:
|
Created Date: 1982-08-07 Completed Date: 1982-08-07 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1942-9 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chromatography, High Pressure Liquid Intestinal Mucosa / metabolism Intestine, Small / metabolism* Male Microsomes, Liver / metabolism Rats Rats, Inbred Strains Tretinoin / analogs & derivatives*, metabolism Vitamin A Deficiency / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
AM 26535/AM/NIADDK NIH HHS; GM 27506/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
13100-69-1/5,6-epoxyretinoic acid; 302-79-4/Tretinoin; 81164-05-8/5,6-epoxyretinoyl glucuronide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Electron uptake and delivery sites on plastocyanin in its reactions with the photosynthetic electron...
Next Document: Physical and biochemical characterization of a purified arginyl-tRNA synthetase-lysyl-tRNA-synthetas...