Document Detail


Metabolism of 2-acetylaminofluorene in cultured human lymphocytes.
MedLine Citation:
PMID:  3575877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The capacity of lymphocytes from 23 human subjects to metabolize the model carcinogen 2-acetylaminofluorene (AAF) was assessed. These cells metabolized AAF to its 1-, 7- and N-hydroxylated metabolites. Alpha-naphthoflavone and metyrapone exhibited IC50's for all pathways of 0.02 microM and 1 mM, respectively. These data indicate that the same form of cytochrome P450 or forms with similar IC50's are mediating these reactions. No significant difference was observed between lymphocytes from smokers versus non-smokers in the N-hydroxylation of AAF, the initial step in the metabolic activation of this carcinogen. However, lymphocytes from smokers were faster detoxifiers of AAF as seen in their increased capacity to 1- and 7-hydroxylate AAF compared to lymphocytes from non-smokers.
Authors:
M E McManus; K J Trainor; A A Morley; W Burgess; I Stupans; D J Birkett
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Research communications in chemical pathology and pharmacology     Volume:  55     ISSN:  0034-5164     ISO Abbreviation:  Res. Commun. Chem. Pathol. Pharmacol.     Publication Date:  1987 Mar 
Date Detail:
Created Date:  1987-06-12     Completed Date:  1987-06-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0244734     Medline TA:  Res Commun Chem Pathol Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  409-18     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
2-Acetylaminofluorene / metabolism*
Biotransformation
Cells, Cultured
Cytochrome P-450 Enzyme System / metabolism
Humans
Hydroxyacetylaminofluorene / metabolism,  pharmacology
Lymphocytes / enzymology,  metabolism*
Metyrapone / pharmacology
Chemical
Reg. No./Substance:
53-95-2/Hydroxyacetylaminofluorene; 53-96-3/2-Acetylaminofluorene; 54-36-4/Metyrapone; 9035-51-2/Cytochrome P-450 Enzyme System

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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