Document Detail


Metabolically convertible lipophilic derivatives of pH-sensitive amphipathic photosensitizers.
MedLine Citation:
PMID:  8309999     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We propose the use of acetoxymethyl esters of pH-sensitive amphipathic photosensitizers (PS) for photodynamic therapy (PDT). These compounds may be applicable for PDT involving endocytosis of lipophilic carriers leading to lysosomal uptake of the esterified PS by target cells. Partial and/or total enzymatic de-esterification may result in the extralysosomal distribution of the photoactive agents, possibly culminating in a multisite photochemical response. We report here the synthesis and properties of chlorin e6 triacetoxymethyl ester (CAME) and pheophorbide a acetoxymethyl ester (PAME). Chlorin e6 and pheophorbide a are photocytotoxic chlorins that possess free carboxylate groups and exhibit optimum wavelengths of excitation substantially red shifted relative to hematoporphyrin derivative. Acetoxymethyl esterification of chlorin e6 and pheophorbide a was accomplished with bromomethyl acetate. High-performance liquid chromatography allowed for the purification of PAME, in 87% purity, and CAME, in 63% yield and 94% purity, as well as the detection of the presumed mono- and diesters of chlorin e6 as transient intermediates in the synthesis of CAME. The ultraviolet-visible absorption, fluorescence excitation and emission, NMR and mass spectra of the chlorin e6 triester are consistent with those expected for CAME. The pH-sensitive amphipathicity of pheophorbide a and chlorin e6 but not CAME was demonstrated using a water/1-octanol partition assay. The production of pheophorbide a from PAME and the sequential formation of the di- and monoesters and free chlorin e6 from CAME, by the action of lysosomal esterases obtained from cancer cells, demonstrate the potential of cellular enzymes to convert the lipophilic esters to pH-sensitive amphipathic PS.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
D Sahai; J L Lo; I K Hagen; L Bergstrom; S Chernomorsky; R D Poretz
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  58     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-03-16     Completed Date:  1994-03-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  803-8     Citation Subset:  IM    
Affiliation:
Biochemistry/FOMRP, Rutgers University, Piscataway, NJ 08855.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma / enzymology
Chlorophyll / analogs & derivatives*,  chemistry,  metabolism
Esterases / metabolism
Esterification
Esters / chemistry
Humans
Hydrogen-Ion Concentration
Lysosomes / enzymology
Photosensitizing Agents / chemistry*
Porphyrins / chemistry*
Solubility
Spectrometry, Fluorescence
Spectrophotometry
Urinary Bladder Neoplasms / enzymology
Chemical
Reg. No./Substance:
0/Esters; 0/Photosensitizing Agents; 0/Porphyrins; 1406-65-1/Chlorophyll; 15664-29-6/pheophorbide a; 19660-77-6/chlorin e6; EC 3.1.-/Esterases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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