| Metabolic viability ofE.coli trapped by dielectrophoresis in microfluidics. | |
| | |
MedLine Citation:
|
PMID: 23175163 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The spatial and temporal control of biological species is essential in complex microfluidic biosystems. In addition, if the biological species is a cell, microfluidic handling must ensure that the cell's metabolic viability is maintained. The use of dielectrophoresis (DEP) for cell manipulation in microfluidics has many advantages since it is remote, fast and the voltages required for cell trapping scale well with miniaturization. In this paper, the conditions for bacterial cell (E.coli) trapping using a quadrupole electrode configuration in apolydimethylsiloxanemicrofluidic channel were developed both for stagnant and for in-flow fluidic situations. The effect of the electrical conductivity of the fluid, the applied electric field and frequency, and the fluid flow velocity were studied. A dynamic exchange between captured and free flowing cells during DEP trapping was demonstrated. The metabolic activity of trapped cells was confirmed by using E. coli cells genetically engineered to express Green Fluorescent Protein (GFP) under the control of an inducible promoter. Non-induced cells trapped by nDEP and pDEP were able to express GFP minutes after the inducer was inserted in the microchannel system immediately after DEP trapping. Longer times of trapping prior to exposure to the inducer indicated first a degradation of the cell metabolic activity and finally cell death. |
| | |
Authors:
|
S S Donato; V Chu; D M F Prazeres; J P Conde |
Related Documents
:
|
23515613 - Erk phosphorylation is predictive of resistance to igf-1r inhibition in small cell lung... 23454523 - Nanoparticle translocation across mouse alveolar epithelial cell monolayers: species-sp... 1290663 - Three-dimensional visualization of renal cells by naoh maceration. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-11-23 |
Journal Detail:
|
Title: Electrophoresis Volume: - ISSN: 1522-2683 ISO Abbreviation: Electrophoresis Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-11-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
|
INESC Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Lisbon, Portugal; IBB - Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Lisbon, Portugal. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Stable and recyclable Au(25) clusters for the reduction of 4-nitrophenol.
Next Document: Evaluation of Intra-Organisational Effects after Use of Certification Programmes in Hospital.