Document Detail


Metabolic viability ofE.coli trapped by dielectrophoresis in microfluidics.
MedLine Citation:
PMID:  23175163     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The spatial and temporal control of biological species is essential in complex microfluidic biosystems. In addition, if the biological species is a cell, microfluidic handling must ensure that the cell's metabolic viability is maintained. The use of dielectrophoresis (DEP) for cell manipulation in microfluidics has many advantages since it is remote, fast and the voltages required for cell trapping scale well with miniaturization. In this paper, the conditions for bacterial cell (E.coli) trapping using a quadrupole electrode configuration in apolydimethylsiloxanemicrofluidic channel were developed both for stagnant and for in-flow fluidic situations. The effect of the electrical conductivity of the fluid, the applied electric field and frequency, and the fluid flow velocity were studied. A dynamic exchange between captured and free flowing cells during DEP trapping was demonstrated. The metabolic activity of trapped cells was confirmed by using E. coli cells genetically engineered to express Green Fluorescent Protein (GFP) under the control of an inducible promoter. Non-induced cells trapped by nDEP and pDEP were able to express GFP minutes after the inducer was inserted in the microchannel system immediately after DEP trapping. Longer times of trapping prior to exposure to the inducer indicated first a degradation of the cell metabolic activity and finally cell death.
Authors:
S S Donato; V Chu; D M F Prazeres; J P Conde
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-23
Journal Detail:
Title:  Electrophoresis     Volume:  -     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
INESC Microsistemas e Nanotecnologias and IN-Institute of Nanoscience and Nanotechnology, Lisbon, Portugal; IBB - Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Lisbon, Portugal.
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