Document Detail


Metabolic and ultrastructural characterization of guinea pig alveolar type II cells isolated by centrifugal elutriation.
MedLine Citation:
PMID:  3718999     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Direct biochemical studies of the whole lung have been quite misleading because of the heterogeneity of the lung cell types. One of the advantages of studying the isolated cells is to be able to correlate specific metabolic functions with intracellular molecular events and to differentiate factors that affect the type II cell function directly. In the present study we have isolated type II cells from guinea pig lung with elastase and purified them by centrifugal elutriation. These cells fluoresce with phosphine 3R as the dye is specifically taken up by the lamellar bodies. In the electron micrographs, the type II cells display punctate villi, which underwent fragmentation in those cases where metrizamide density gradient was used. Mitochondria are scattered throughout the cytoplasm, and smooth endoplasmic reticulum is sparse. Type II cells possess large irregularly shaped nuclei with peripheral areas of dense hemochromatin and at least one prominent nucleolus. Ovoid lamellar bodies are the most prominent cellular inclusions. These bodies are present throughout the cytoplasm and contain a substructure of whorling and concentric laminations. Biochemical studies indicate that type II cells prepared by centrifugal elutriation are metabolically well preserved as seen from incorporation of [14C]leucine into cellular proteins, [methyl-14C]choline into cellular disaturated phosphatidylcholine and CDP[methyl-14C]choline into mitochondrial and microsomal phosphatidylcholine. Superiority of centrifugal elutriation over the commonly employed combination of discontinuous metrizamide gradient and cell elutriation is evident from the present study.
Authors:
M O Sikpi; C R Nair; A E Johns; S K Das
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  877     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1986 Jun 
Date Detail:
Created Date:  1986-07-28     Completed Date:  1986-07-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  20-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation / methods
Centrifugation, Density Gradient
Guinea Pigs
Male
Metrizamide
Microscopy, Electron
Microscopy, Fluorescence
Pulmonary Alveoli / cytology*
Grant Support
ID/Acronym/Agency:
RR-08037/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
31112-62-6/Metrizamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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