| Metabolic and structural rearrangement during dark-induced autophagy in soybean (Glycine max L.) nodules: an electron microscopy and 31P and 13C nuclear magnetic resonance study. | |
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MedLine Citation:
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PMID: 20358222 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of dark-induced stress on the evolution of the soluble metabolites present in senescent soybean (Glycine max L.) nodules were analysed in vitro using (13)C- and (31)P-NMR spectroscopy. Sucrose and trehalose were the predominant soluble storage carbons. During dark-induced stress, a decline in sugars and some key glycolytic metabolites was observed. Whereas 84% of the sucrose disappeared, only one-half of the trehalose was utilised. This decline coincides with the depletion of Gln, Asn, Ala and with an accumulation of ureides, which reflect a huge reduction of the N(2) fixation. Concomitantly, phosphodiesters and compounds like P-choline, a good marker of membrane phospholipids hydrolysis and cell autophagy, accumulated in the nodules. An autophagic process was confirmed by the decrease in cell fatty acid content. In addition, a slight increase in unsaturated fatty acids (oleic and linoleic acids) was observed, probably as a response to peroxidation reactions. Electron microscopy analysis revealed that, despite membranes dismantling, most of the bacteroids seem to be structurally intact. Taken together, our results show that the carbohydrate starvation induced in soybean by dark stress triggers a profound metabolic and structural rearrangement in the infected cells of soybean nodule which is representative of symbiotic cessation. |
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Authors:
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Pierre Vauclare; Richard Bligny; Elisabeth Gout; Valentine De Meuron; Fran?ois Widmer |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-01 |
Journal Detail:
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Title: Planta Volume: 231 ISSN: 1432-2048 ISO Abbreviation: Planta Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-14 Completed Date: 2010-06-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1250576 Medline TA: Planta Country: Germany |
Other Details:
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Languages: eng Pagination: 1495-504 Citation Subset: IM |
Affiliation:
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Laboratory of Plant Biology and Physiology, Biology Building UNIL, Room 5449, 1015 Lausanne, Switzerland. pierre.vauclare@wanadoo.fr |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Autophagy* Carbon Isotopes Cell Aging Cell Membrane / metabolism Darkness* Fatty Acids / metabolism Magnetic Resonance Spectroscopy Metabolome Microscopy, Electron Phosphorus Isotopes Root Nodules, Plant / cytology, metabolism*, ultrastructure* Soybeans / cytology, metabolism*, ultrastructure* Stress, Physiological |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Fatty Acids; 0/Phosphorus Isotopes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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