Document Detail

Metabolic relationship between arachidonate activation and its transfer to lysophospholipids by brain microsomes.
MedLine Citation:
PMID:  3934574     Owner:  NLM     Status:  MEDLINE    
Evidence is presented to indicate a metabolic relationship between arachidonic acid activation and its transfer to lysophospholipids by brain microsomes. Thus, in the presence of 1-acylglycerophosphocholines or 1-acyl-glycerophosphoinositols, the activation of labeled arachidonate to its acyl-CoA was enhanced, and the acyl-CoA formed was, in turn, transferred to the lysophospholipids to form the respective diacyl-glycerophospholipids. The "coupling effect" seems to pertain mainly to the lysophospholipids which are good substrates of the acyltransferase. Other lyso-compounds were either not effective or inhibitory to the arachidonate activation process. The activation-transfer activity mediated by the fatty acid ligase and acyltransferase could be dissociated by Triton X-100, which apparently stimulated the acyl-CoA ligase activity but inhibited the acyltransferase. These results suggest that fatty acid ligase and acyltransferase are located in close proximity within the membrane domain. The existence of a close metabolic relationship between these two enzymic reactions is important for maintaining a dynamic equilibrium between the free fatty acids and the membrane phospholipids. The mechanism is also useful in regulating the cellular acyl-CoA and lysophospholipid metabolism, because both compounds have membrane perturbing properties when present in excessive quantity.
W Tang; G Y Sun
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Neurochemical research     Volume:  10     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1986-01-16     Completed Date:  1986-01-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1343-53     Citation Subset:  IM    
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MeSH Terms
Acyl Coenzyme A / metabolism
Adenosine Triphosphate / metabolism
Arachidonic Acid
Arachidonic Acids / metabolism*
Brain / metabolism*
Fatty Acids / metabolism
Magnesium / metabolism
Microsomes / metabolism*
Phospholipids / biosynthesis,  metabolism*
Rats, Inbred Strains
Time Factors
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Arachidonic Acids; 0/Fatty Acids; 0/Phospholipids; 506-32-1/Arachidonic Acid; 56-65-5/Adenosine Triphosphate; 7439-95-4/Magnesium

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