Document Detail


Metabolic regulation of coronary vascular tone: role of endothelin-1.
MedLine Citation:
PMID:  12384469     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Coronary tone is determined by a balance between endogenously produced endothelin and metabolic dilators. We hypothesized that coronary vasodilation during augmented metabolism is the net result of decreased endothelin production and increased production of vasodilators. Isolated rat myocytes were stimulated at 0, 200, and 400 beats/min to modify metabolism. Supernatant from these preparations was added to isolated coronary arterioles with and without blocking vasoactive pathways (adenosine, bradykinin, and endothelin). Chronically instrumented swine were studied while resting and running on a treadmill before and after endothelin type A (ET(A)) receptor blockade. The vasodilatory properties of the supernatant increased with increased stimulation frequencies. Combined blockade of adenosine and bradykinin receptors abolished vasodilation in response to supernatant of stimulated myocytes. ET(A) blockade increased vasodilation to supernatant of unstimulated myocytes but did not affect dilation to supernatant of myocytes stimulated at 400 beats/min. In vivo, ET(A) blockade resulted in coronary vasodilation at rest, which waned during exercise. Thus endothelin has a tonic constrictor influence through the ET(A) receptor at low myocardial metabolic demand but its influence decreased during increased metabolism.
Authors:
Daphne Merkus; Dirk J Duncker; William M Chilian
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  283     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-10-17     Completed Date:  2002-11-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1915-21     Citation Subset:  IM    
Affiliation:
Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53227, USA. merkus@tch.fgg.eur.nl
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MeSH Terms
Descriptor/Qualifier:
Animals
Coronary Circulation / physiology*
Coronary Vessels / metabolism*
Endothelin-1 / metabolism*
Female
Male
Microcirculation / physiology
Myocytes, Cardiac / metabolism
Oxygen Consumption / physiology
Rats
Rats, Wistar
Receptor, Endothelin A
Receptors, Endothelin / metabolism
Swine
Vasoconstriction / physiology
Vasodilation / physiology
Grant Support
ID/Acronym/Agency:
HL 32788/HL/NHLBI NIH HHS; HL 65203/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Endothelin-1; 0/Receptor, Endothelin A; 0/Receptors, Endothelin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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