| METABOLIC PROPERTIES OF CELLS ISOLATED FROM ADULT MOUSE LIVER. | |
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MedLine Citation:
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PMID: 19866607 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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Suspensions of isolated cells were prepared from mouse livers that had been perfused via the portal vein with a buffered medium containing sucrose. The demonstration of metabolic activities in these cells was found to be critically dependent on the composition of the suspending medium. The cells showed considerable metabolic activity in a simple medium containing 0.06 to 0.20 M sucrose, but did not respire in 0.30 M sucrose medium. Endogenous respiration was greatest when the sucrose concentration of the medium was 0.10 M or lower and was associated with the formation of acetoacetate. The cells oxidized citric acid cycle intermediates, glutamate, lactate, pyruvate, beta-hydroxybutyrate, alpha-glycerophosphate, and fatty acids and synthesized urea from ammonium chloride, but carbohydrate substrates did not stimulate oxygen uptake. Cells incubated in Krebs' phosphate-saline did not respire. The lack of respiration in this medium is thought to be related to increased permeability of the cell membrane with penetration of calcium ions and orthophosphate into the cells causing mitochondrial swelling and destruction. Further evidence for the loss of cellular permeability barriers is provided by the demonstration of leakage of certain soluble enzymes into the preparative media. |
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Authors:
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M N Berry |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of cell biology Volume: 15 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1962 Oct |
Date Detail:
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Created Date: 2010-06-22 Completed Date: 2010-06-22 Revised Date: 2010-09-28 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: United States |
Other Details:
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Languages: eng Pagination: 1-8 Citation Subset: - |
Affiliation:
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Department of Biochemistry, Medical School, University of Otago, Dunedin, New Zealand. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © Copyright, 1962, by The Rockefeller Institute Press Received Day: 28 Month: 3 Year: 1962 Print publication date: Day: 1 Month: 10 Year: 1962 Volume: 15 Issue: 1 First Page: 1 Last Page: 8 ID: 2106128 PubMed Id: 19866607 |
| METABOLIC PROPERTIES OF CELLS ISOLATED FROM ADULT MOUSE LIVER | |
| M. N. Berry | |
| From the Department of Biochemistry, Medical School, University of Otago, Dunedin, New Zealand. |
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| Dr. Berry's present address is Department of Biochemistry, Oxford, England |
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